Woronin bodies, their impact on stress resistance and virulence of the pathogenic mould Aspergillus fumigatus and their anchoring at the septal pore of filamentous Ascomycota
Woronin bodies, their impact on stress resistance and virulence of the pathogenic mould Aspergillus fumigatus and their anchoring at the septal pore of filamentous Ascomycota
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DOI：
10.1111/mmi.12316
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发表时间：
2013-09-01
影响因子：
3.6
通讯作者：
Ebel, Frank
中科院分区：
文献类型：
--
作者：
Beck, Julia;Echtenacher, Bernd;Ebel, Frank
丝状子囊菌门的菌丝由隔室组成，隔室通过隔膜孔连接。为了避免受伤后细胞内容物的急剧损失，真菌开发了封闭其隔膜孔的机制。在大多数盘菌亚门中，所谓的Woronin体锚定在孔附近。这是细胞器精确空间定位的一个突出例子，但到目前为止，基本的分子组织仍然是未知的。使用致病性霉菌烟曲霉，我们提供的证据表明，Woronin机构是重要的抗逆性和毒力。此外，分子机械锚定他们在隔膜描述。也就是说，我们已经确定了拉作为拴系蛋白，并提供证据表明，Woronin体蛋白HexA结合到隔膜孔在拉依赖的方式。此外，我们证明了一个引人注目的多组氨酸基序的目标六角到中隔细胞壁。因此，HexA-Lah轴是连接Woronin体与中隔的系绳的极佳候选者。这种模式适用于烟曲霉，但也很可能适用于绝大多数盘菌亚门。我们的研究结果揭示了Woronin身体锚定的演变，并为开发新的策略，以打击真菌病原体，如A. fumigatus的基础。
Hyphae of filamentous Ascomycota consist of compartments that are connected via septal pores. To avoid a dramatic loss of cellular content after wounding, fungi developed mechanisms to occlude their septal pores. In most Pezizomycotina, so-called Woronin bodies are anchored in proximity to the pore. This is a prominent example for precise spatial positioning of organelles, but so far the underlying molecular organization has remained largely unknown. Using the pathogenic mould Aspergillus fumigatus, we provide evidence that Woronin bodies are important for stress resistance and virulence. Furthermore the molecular machinery anchoring them at the septum is described. Namely, we have identified Lah as the tethering protein and provide evidence that the Woronin body protein HexA binds to the septal pore in a Lah-dependent manner. Moreover, we demonstrate that a striking poly-histidine motif targets HexA to the septal cell wall. Thus, the axis HexA-Lah is an excellent candidate for the tether linking Woronin bodies to the septum. This model applies to A.fumigatus, but most likely also to the vast majority of the Pezizomycotina. Our findings shed light on the evolution of Woronin body anchoring and provide a basis for the development of novel strategies to combat fungal pathogens like A.fumigatus.