Replication of Avocado Sunblotch Viroid in the Yeast Saccharomyces cerevisiae
Replication of Avocado Sunblotch Viroid in the Yeast Saccharomyces cerevisiae
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DOI：
10.1128/jvi.01320-10
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发表时间：
2011-04-01
影响因子：
5.4
通讯作者：
Torchet, Claire
中科院分区：
文献类型：
--
作者：
Delan-Forino, Clementine;Maurel, Marie-Christine;Torchet, Claire
类病毒是已知最小的病原体。它们是非编码、单链、闭环、“裸”RNA，通过 RNA-RNA 转录进行复制。 Avsunviroidae 家族的类病毒在其序列中具有锤头状核酶，允许在复制过程中进行自我切割。迄今为止，仅在植物细胞中检测到类病毒。在这里，我们研究了 Avsunviroidae 家族的鳄梨日斑类病毒 (ASBVd) 在非常规宿主——酿酒酵母中的复制。我们证明两种极性的 ASBVd RNA 链都能够在单细胞真核细胞中自我切割和复制。我们发现类病毒单体 RNA 因核 3' 和细胞质 5' RNA 降解途径而不稳定。我们的结果首次提供证据表明类病毒可以在植物以外的其他生物体中复制，并且酵母含有 ASBVd 复制的所有必需细胞元件。
Viroids are the smallest known pathogenic agents. They are noncoding, single-stranded, closed-circular, "naked" RNAs, which replicate through RNA-RNA transcription. Viroids of the Avsunviroidae family possess a hammerhead ribozyme in their sequence, allowing self-cleavage during their replication. To date, viroids have only been detected in plant cells. Here, we investigate the replication of Avocado sunblotch viroid (ASBVd) of the Avsunviroidae family in a nonconventional host, the yeast Saccharomyces cerevisiae. We demonstrate that ASBVd RNA strands of both polarities are able to self-cleave and to replicate in a unicellular eukaryote cell. We show that the viroid monomeric RNA is destabilized by the nuclear 3' and the cytoplasmic 5' RNA degradation pathways. For the first time, our results provide evidence that viroids can replicate in other organisms than plants and that yeast contains all of the essential cellular elements for the replication of ASBVd.