Analysis of the tobacco-specific nitrosamine 4-(methyinitrosamino)-1-(3-pyridyl)-1-butanol in urine by extraction on a molecularly imprinted polymer column and liquid chromatography/atmospheric pressure ionization tandem mass spectrometry
Analysis of the tobacco-specific nitrosamine 4-(methyinitrosamino)-1-(3-pyridyl)-1-butanol in urine by extraction on a molecularly imprinted polymer column and liquid chromatography/atmospheric pressure ionization tandem mass spectrometry
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DOI：
10.1021/ac058027u
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发表时间：
2005-12-01
影响因子：
7.4
通讯作者：
Bernert, JT
中科院分区：
文献类型：
--
作者：
Xia, Y;McGuffey, JE;Bernert, JT
烟草特有的亚硝胺4-（甲基亚硝胺基）1-（3-吡啶基）-1-丁醇（NNAL）存在于烟草使用者的尿液中，并且以较低的浓度存在于暴露于二手烟的非吸烟者的尿液中。NNAL是人类暴露于烟草和烟草烟雾中致癌亚硝胺的有价值的生物标志物，但其在尿液中的低浓度存在需要敏感且通常复杂的分析程序。在这份报告中，我们描述了一种有效的方法，用于分析NNAL在人类尿液中使用液相色谱/大气压电离串联质谱（LC/MS/MS）结合一种新的样品净化的基础上，分子印迹聚合物（MIP）柱专门为此检测开发的开发。我们的研究结果表明，MIP柱提取和ILC/MS/MS的组合可以提供一个敏感和相对简单的分析方法，适用于吸烟者和非吸烟者暴露于烟草烟雾或SHS的健康风险的流行病学调查。
The tobacco-specific nitrosamine 4-(methylnitrosamino)1-(3-pyridyl)-1-butanol (NNAL) is present in the urine of tobacco users and, at lower concentrations, in the urine of nonsmokers exposed to secondhand smoke. NNAL is a valuable biomarker of human exposure to the carcinogenic nitrosamines in tobacco and tobacco smoke, but its presence at low concentrations in urine requires sensitive and often complex analytic procedures. In this report, we describe the development of an efficient method for the analysis of NNAL in human urine using liquid chromatography/atmospheric pressure ionization tandem mass spectrometry (LC/MS/MS) combined with a novel sample cleanup based on a molecularly imprinted polymer (MIP) column developed specifically for this assay. Our results suggest that this combination of MIP column extraction and ILC/MS/MS can provide a sensitive and relatively simple analytical method suitable for application to epidemiologic investigations of health risks associated with the exposure to tobacco smoke or SHS in both smokers and nonsmokers.