Inositol Hexakisphosphate and Inositol Enhance the Inhibition of Colorectal Cancer Growth and Liver Metastasis by Capecitabine in a Mouse Model
Inositol Hexakisphosphate and Inositol Enhance the Inhibition of Colorectal Cancer Growth and Liver Metastasis by Capecitabine in a Mouse Model
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DOI：
10.1080/01635581.2020.1820055
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发表时间：
2020-09-15
影响因子：
2.9
通讯作者：
Song, Yang
中科院分区：
文献类型：
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作者：
Li, Chunlei;Ci, Yifan;Song, Yang
为探讨六磷酸肌醇(IP6)和肌醇(INS)联合卡培他滨对小鼠结直肠癌(CRC)生长和肝转移的影响，建立了小鼠结直肠癌原位移植模型。实验分为5组：对照组、模型组、卡培他滨(60 mg/kg)组、IP6+INS(80 mg/kg：80 mg/kg)组、卡培他滨+IP6+INS(60 mg/kg：80 mg/kg：80 mg/kg)组。与模型组相比，其他3个治疗组的肿瘤参数均显著降低。IP6、INS与卡培他滨联合应用在提高生存率、减轻肿瘤重量、抑制肝转移方面更有效。与模型组比较，各治疗组E-钙粘蛋白表达上调，N-钙粘蛋白和波形蛋白表达抑制。这一现象在联合组中更为明显。与其他干预组相比，联合用药显著降低了结直肠癌小鼠血清中肿瘤坏死因子-α、白介素6和白介素8的表达水平。我们的数据表明，IP6和INS通过调节炎症因子、细胞间黏附分子和波形蛋白的表达来增强卡培他滨对小鼠结直肠癌生长的影响。
To investigate the effects of inositol hexaphosphate (IP6) and inositol (INS) with capecitabine treatment on colorectal cancer (CRC) growth and liver metastasis in mice, we established an orthotopic xenograft mouse model. The study designated five experimental groups: a control group, a model group, a capecitabine (60 mg/kg) treatment group, an IP6 + INS (80 mg/kg: 80 mg/kg) treatment group, and a capecitabine + IP6 + INS (60 mg/kg: 80 mg/kg: 80 mg/kg) treatment group. Compared with the model group, the tumor parameters of the other three treatment groups were significantly reduced. The combination of IP6 and INS with capecitabine is more effective in improving survival rate, reducing tumor weight, and inhibiting liver metastasis. Compared with the model group, the expression of E-cadherin in each treatment group was elevated, while the expression of N-cadherin and vimentin was suppressed. This phenomenon was more obvious in the combination group. The combination more significantly reduced the expression levels of TNF-alpha, IL-6, and IL-8 in the serum of CRC mice compared with other intervention groups. Our data indicate that IP6 and INS enhanced the effect of capecitabine on CRC growth in mice by modulating the expression of inflammatory factors, intercellular adhesion molecules, and vimentin.