Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family
Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family
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DOI：
10.1093/oxfordjournals.molbev.a025894
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发表时间：
1998-12-01
影响因子：
10.7
通讯作者：
Baker, RJ
中科院分区：
文献类型：
--
作者：
Nekrutenko, A;Hillis, DM;Baker, RJ
在这项研究中，我们报道了人类、小鼠以及两种田鼠（墨西哥田鼠和草原田鼠）的胞质依赖NADP的异柠檬酸脱氢酶的cDNA序列。从这些分类群推断出的氨基酸序列显示出高度的氨基酸序列保守性，与肌球蛋白β重链相当，并且具有已知的结构特征。一种秀丽隐杆线虫的酶，先前被鉴定为与异柠檬酸脱氢酶相似的蛋白质，根据其与哺乳动物酶的氨基酸相似性和系统发育分析，很可能是等同于依赖NADP的胞质异柠檬酸脱氢酶的酶。我们还认为，从苜蓿、大豆和桉树中鉴定出的依赖NADP的异柠檬酸脱氢酶很可能是胞质酶。来自真核生物的各种异柠檬酸脱氢酶的系统发育树显示，独立的基因重复可能导致了动物和真菌中依赖NADP的异柠檬酸脱氢酶的胞质和线粒体形式的产生。对于该酶的线粒体形式和胞质形式在这些类群中是直系同源的这一假设，似乎没有统计支持。提出了一种依赖NADP的异柠檬酸脱氢酶进化的可能情形。
In this study, we report cDNA sequences of the cytosolic NADP-dependent isocitrate dehydrogenase for humans, mice, and two species of voles (Microtus mexicanus and Microtus ochrogaster). inferred amino acid sequences from these taxa display a high level of amino acid sequence conservation, comparable to that of myosin beta heavy chain, and share known structural features. A Caenorhabditis: elegans enzyme that was previously identified as a protein similar to isocitrate dehydrogenase is most likely the NADP-dependent cytosolic isocitrate dehydrogenase enzyme equivalent, based on amino acid similarity to mammalian enzymes and phylogenetic analysis. We also suggest that NADP-dependent isocitrate dehydrogenases characterized from alfalfa, soybean, and eucalyptus are most likely cytosolic enzymes. The phylogenetic tree of various isocitrate dehydrogenases from eukaryotic sources revealed that independent gene duplications may have given rise to the cytosolic and mitochondrial forms of NADP-dependent isocitrate dehydrogenase in animals and fungi. There appears to be no statistical support for a hypothesis that the mitochondrial and cytosolic forms of the enzyme are orthologous in these groups. A possible scenario of the evolution of NADP-dependent isocitrate dehydrogenases is proposed.