Immunohistological Study of the Major Salivary Glands in the Gray Short-Tailed Opossums (Monodelphis domestica)
Immunohistological Study of the Major Salivary Glands in the Gray Short-Tailed Opossums (Monodelphis domestica)
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灰短尾负鼠（Monodelphis Domestica）主要唾液腺的免疫组织学研究
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
I. Nasu
中科院分区：
文献类型：
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作者：
Hiroya Gotouda;R. Tamamura;Tetsuro Kono;Yukari Ootani;Takeshi Kanno;Takao Kuwada;Kunihiro Suzuki;T. Sakae;H. Okada;I. Nasu
有袋动物是哺乳动物，其幼崽出生在未发育阶段，并附在母亲的乳头上，通常在育儿袋中。尽管如此，这些颚部元素对骨骼和消化系统必须早期发育。我们以前曾报道过灰短尾负鼠唾液腺的形态。这种负鼠是一种有袋类动物，有三个大的唾液腺，包括腮腺、下颌下腺和舌下腺。腮腺仅由浆液性腺泡细胞组成。颌下腺和舌下腺均为浆粘液分泌终片，为混合腺。本研究旨在探讨细胞角蛋白19（CK19）、水通道蛋白5（AQP5）和α平滑肌肌动蛋白（SMA）在灰短尾负鼠腮腺、颌下腺和舌下腺中的定位。这些蛋白质的定位通过石蜡切片上的免疫组织化学确定。下颌下腺和舌下腺显示出大致相似的染色模式。CK19定位于腺泡细胞和导管细胞在三个主要的唾液腺。AQP5仅在浆膜腔表达。α-SMA被观察到周围的分泌结束件。这些结果被认为反映了负鼠唾液腺的特征。哺乳动物唾液腺的细胞分化和生理功能尚需进一步研究。
Marsupials were mammals whose young were born in an undeveloped stage and attached to the mother’s nipples, usually in a pouch. Despite, these jaw elements to suckle and digestive system must develop early. Previously we have reported that the morphology of the salivary glands in the gray short-tailed opossums (Monodelphis domestica). This opossum, a kind of marsupial, has three large salivary glands which include the parotid, submandibular and sublingual glands. Parotid glands consist only of serous acinar cells. Submandibular and sublingual glands consist of seromucous secretory end pieces and the submandibular and sublingual glands were mixed glands. This study was purpose to investigate the localization of cytokeratin19 (CK19), aquaporin5 (AQP5) and alpha smooth muscle actin (SMA) in the gray short-tailed opossum parotid, submandibular and sublingual glands. These proteins localization was determined by immunohistochemistry on paraffin sections. Submandibular and sublingual glands showed a broadly similar staining pattern. CK19 was localized to the acinar cells and ductal cells in three major salivary glands. AQP5 was expressed in the luminal only of serous cells. Alpha-SMA was observed around the secretory end pieces. These results are thought to reflect the characteristic of the opossum salivary glands. Further studies are required to unravel obscure matters with cell differentiation and physiological functions of salivary glands among the mammals.