Carriers in mesenchymal stem cell osteoblast mineralization-State-of-the-art
Carriers in mesenchymal stem cell osteoblast mineralization-State-of-the-art
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DOI：
10.1016/j.jcms.2013.01.047
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发表时间：
2014-01-01
影响因子：
3.1
通讯作者：
Pinholt, Else Marie
中科院分区：
文献类型：
--
作者：
Dahl, Morten;Jorgensen, Niklas Rye;Pinholt, Else Marie
目的：组织工程是将具有成骨能力的细胞与合适的支架材料联合收割机结合，实现骨组织再生的一种新方法。我们的目的是在Medline搜索中评估成骨细胞矿化在体外和体内，包括基因表达结合间充质干细胞（MSC）和五种不同的载体，钛，胶原蛋白，碳酸钙，磷酸钙和聚乳酸-聚乙醇酸共聚物的meta-或描述性分析的目的。材料和方法：搜索包括以下MeSH单词的不同组合-间充质干细胞，碱性磷酸酶，骨再生，组织工程，药物载体，组织支架，钛，胶原蛋白，碳酸钙，结果：80篇文献中有2篇包括数值和作为对照的载体和细胞对矿化和基因表达的影响。β-磷酸三钙（β-TCP）显示升高的碱性磷酸酶活性，和钙缺乏的羟基磷灰石一个更大的基因表达的骨钙素时，接种诱导MSCs.Conclusion：没有数据发表钛作为载体在MSC成骨细胞矿化。荟萃分析和描述性分析分别包括试验材料和载体和细胞对照反应的数值。只有两篇文章符合这些要求。(C)2013年欧洲颅颌面外科协会。由爱思唯尔有限公司出版。保留所有权利。
Purpose: Tissue engineering is a new way to regenerate bone tissue, where osteogenic capable cells combine with an appropriate scaffolding material. Our aim was in a Medline Search to evaluate osteoblast mineralization in vitro and in vivo including gene expressing combining mesenchymal stem cells (MSCs) and five different carriers, titanium, collagen, calcium carbonate, calcium phosphate and polylactic acid-polyglycolic acid copolymer for purpose of a meta-or a descriptive analysis.Materials and methods: The search included the following MeSH words in different combinations-mesenchymal stem cells, alkaline phosphatase, bone regeneration, tissue engineering, drug carriers, tissue scaffolds, titanium, collagen, calcium carbonate, calcium phosphates and polylactic acid-polyglycolic acid copolymer.Results: Two out of 80 articles included numerical values and as control, carriers and cells, on mineralization and gene expression. beta-tricalcium phosphate (beta-TCP) revealed elevated alkaline phosphatase activity, and calcium-deficient hydroxyapatite a greater gene expression of osteocalcin when seeded with induced MSCs.Conclusion: No data are published on titanium used as a carrier in MSC osteoblast mineralization. A meta-as well as a descriptive analysis includes numerical values of test materials and of control reactions from carrier and cells, respectively. Only two articles fulfilled these requirements. (C) 2013 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.