Calorie restriction enhances adult mouse lung stem cells function and reverses several ageing-induced changes
Calorie restriction enhances adult mouse lung stem cells function and reverses several ageing-induced changes
复制标题
热量限制增强成年小鼠肺干细胞功能并逆转一些衰老引起的变化
DOI：
10.1002/term.2792
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发表时间：
2019
影响因子：
3.3
通讯作者：
Betsuyaku Tomoko
中科院分区：
文献类型：
--
作者：
Hegab Ahmed E.;Ozaki Mari;Meligy Fatma Y.;Nishino Makoto;Kagawa Shizuko;Ishii Makoto;Betsuyaku Tomoko
老龄化与肺功能下降和肺部感染发病率增加有关。一些研究表明，长期的卡路里限制（CR）可以促进健康和长寿，并降低患多种疾病的风险。CR的作用被认为是通过改善组织干细胞的功能。已知干细胞功能会随着年龄的增长而下降。在这项研究中，我们研究了老化对肺上皮细胞和干细胞的影响，以及CR对年轻和老年肺的影响。我们发现衰老导致气管基底干细胞减少。CR诱导年轻和老年小鼠的基底干细胞增加。此外，老化诱导肺部炎症，CR倾向于减少年轻小鼠的基线肺部炎性细胞浸润，并显著减少老化诱导的肺部炎症。此外，衰老降低了肺线粒体的数量和功能，增加了线粒体活性氧的水平。CR增加了年轻和老年小鼠线粒体的数量和功能。此外，老化降低了肺干细胞集落形成效率（CFE），CR增加了年轻和老年小鼠的CFE。最后，CR改善了年轻小鼠损伤肺中上皮细胞的存活。总之，衰老导致肺上皮细胞的几种结构和功能变化/损伤。CR在肺上皮细胞中诱导几种潜在的有益变化，即使在老年时开始，包括逆转一些衰老诱导的变化。
Ageing is associated with decreased lung function and an increased incidence of lung infections. Several studies have suggested that long‐term calorie restriction (CR) promotes health and longevity and results in the reduced risk of several diseases. The effect of CR is thought to be through improving the function of tissue stem cells. Stem cell function is known to decline with ageing. In this study, we examined the effects of ageing on lung epithelial and stem cells and the effect of CR on young and old lungs. We found that ageing results in a decrease in tracheal basal stem cells. CR induced an increase in basal stem cells in both young and old mice. In addition, ageing induced lung inflammation, and CR tended to reduce baseline lung inflammatory cell infiltration in young mice and significantly reduced ageing‐induced lung inflammation. Furthermore, ageing reduced the number and function of mitochondria in lung and increased the level of mitochondrial reactive oxygen species. CR increased the number and function of mitochondria both in young and old mice. Moreover, ageing reduced lung stem cell colony‐forming efficiency (CFE), and CR increased the CFE in both young and old mice. Finally, CR improved epithelial cell survival in injured lungs of young mice. In conclusion, ageing causes several structural and functional changes/impairments in lung epithelial cells. CR induces several potentially beneficial changes in lung epithelial cells, even when it is initiated at an older age, including reversal of some ageing‐induced changes.