Development of a serogroup-specific DNA microarray for identification of Escherichia coli strains associated with bovine septicemia and diarrhea
Development of a serogroup-specific DNA microarray for identification of Escherichia coli strains associated with bovine septicemia and diarrhea
复制标题
开发血清群特异性 DNA 微阵列，用于鉴定与牛败血症和腹泻相关的大肠杆菌菌株
DOI：
10.1016/j.vetmic.2009.10.019
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发表时间：
2010-05-19
影响因子：
3.3
通讯作者：
Wang, Lei
中科院分区：
文献类型：
--
作者：
Liu, Bin;Wu, Fan;Wang, Lei
属于O8、O9、O15、O26、O35、O78、O86、O101、O115和O119血清群的大肠杆菌菌株通常与犊牛败血症或腹泻有关，对全世界的养牛业构成重大威胁。在本研究中，开发了一种针对o抗原特异性基因的微阵列检测系统，用于鉴定这些血清群。通过对186株大肠杆菌和志贺氏菌o血清组参考菌株、36株大肠杆菌临床分离株和9株其他密切相关菌种的代表性菌株的检测，表明该芯片具有特异性和可重复性。检测灵敏度为50 ng基因组DNA。这里开发的微阵列分析适用于从环境和/或临床样本中检测和鉴定相关菌株，特别适用于流行病学研究。(C) 2009 Elsevier B.V.版权所有
Escherichia coli strains belonging to serogroups O8, O9, O15, O26, O35, O78, O86, O101, O115 and O119 are commonly associated with septicemia or diarrhea in calves and pose a significant threat to the cattle industry worldwide. In this study, a microarray detection system targeting O-antigen-specific genes was developed for the identification of those serogroups. By testing against 186 E. coli and Shigella O-serogroup reference strains, 36 E. coli clinical isolates, and 9 representative strains of other closely related bacterial species, the microarray was shown to be specific and reproducible. The detection sensitivity was determined to be 50 ng genomic DNA. The microarray assay developed here is suitable for the detection and identification of relevant strains from environmental and/or clinical samples, and is especially useful for epidemiologic studies. (C) 2009 Elsevier B.V. All rights reserved.