High-Precision GC-MS Analysis of Atmospheric Polycyclic Aromatic Hydrocarbons (PAHs) and Isomer Ratios from Biomass Burning Emissions
High-Precision GC-MS Analysis of Atmospheric Polycyclic Aromatic Hydrocarbons (PAHs) and Isomer Ratios from Biomass Burning Emissions
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DOI：
10.4236/jep.2011.24051
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发表时间：
2011-06
期刊：
影响因子：
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通讯作者：
Prashant Rajput;M. Sarin;R. Rengarajan
中科院分区：
文献类型：
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作者：
Prashant Rajput;M. Sarin;R. Rengarajan
本文介绍了一种用加速溶剂萃取（ASE）、硅胶柱净化、气相色谱-质谱联用仪（GC-MS）测定16-多环芳烃（PAHs）的分析方法。以二氯甲烷（DCM）为溶剂，优化了萃取条件（T = 100 oC，P = 1500 psi，t = 30 min，V = 30 ml），避免了分馏效应，实现了多环芳烃的定量质量回收。硅胶柱净化多环芳烃消除了基体效应，有利于多环芳烃从萃取液中富集，并以Pyrene-D_（10）为内标进行定量测定。分析协议已成功地用于量化的16-多环芳烃及其异构体的比例在大气气溶胶收集从印度北方占主导地位的农业废物（收获后的水稻和小麦残留物）燃烧排放。根据对不同地点收集的大气气溶胶的分析，2- 3环多环芳烃的总回收率为85%，4- 6环化合物的回收率接近100%。
This manuscript describes an analytical method for the quantitative determination of 16-polycyclic aromatic hydrocarbons (PAHs) using accelerated solvent extraction (ASE), followed by purification on a silica cartridge, and subsequent measurement by gas chromatograph coupled to a mass spectrometer (GC-MS). The solvent extraction parameters (T = 100 oC, P = 1500 psi, t = 30 min, V = 30 ml) are optimized with dichloromethane (DCM) in order to avoid fractionation effect, thereby achieving quantitative mass recovery of PAHs. The purification of PAHs on silica cartridge eliminates the matrix effect, facilitates their enrichment from extracted solution and quantitative deter-mination in presence of an internal-standard (Pyrene-D10). The analytical protocol has been successfully used for the quantification of 16-PAHs and their isomer ratios in atmospheric aerosols collected from northern India dominated by agricultural- waste (post-harvest paddy and wheat residue) burning emissions. Based on the analysis of ambient aerosols, collected from different sites, the overall recovery efficiency for 2- to 3-ring PAHs is 85% and near 100% recovery for 4- to 6-ring compounds.