VARIATIONS IN SUSCEPTIBILITY OF FIELD AND LABORATORY POPULATIONS OF CULEX-TARSALIS TO EXPERIMENTAL-INFECTION WITH WESTERN EQUINE ENCEPHALOMYELITIS VIRUS
VARIATIONS IN SUSCEPTIBILITY OF FIELD AND LABORATORY POPULATIONS OF CULEX-TARSALIS TO EXPERIMENTAL-INFECTION WITH WESTERN EQUINE ENCEPHALOMYELITIS VIRUS
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DOI：
10.1093/oxfordjournals.aje.a112251
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发表时间：
1976-01-01
影响因子：
5
通讯作者：
SJOGREN, RD
中科院分区：
文献类型：
--
作者：
HARDY, JL;REEVES, WC;SJOGREN, RD
对来自美国加州的4个野外种群和4个实验室群体进行了胸腔接种和取食浸泡在病毒-血液-蔗糖混合物中的棉球的方法，以研究它们对西方马脑脊髓炎病毒感染的敏感性。所有品系的蚊子均对胸腔接种感染敏感，但野外品系和定植品系的50%感染剂量分别相差1000-1500倍。用棉球饲养获得的剂量-反应曲线表明，一些现场株和实验室株的敏感度存在很大的异质性，因为有些个体雌性可以在摄取小浓度的病毒后感染，而另一些则不能通过增加1000-10000倍的病毒浓度而感染。不同蚊种对病毒敏感性的差异与自生率或有机磷杀虫剂抗药性的差异不相关。
Four field populations and 4 laboratory colonies of Culex tarsalis from California [USA] were evaluated for their susceptibility to infection with western equine encephalomyelitis (WEE) virus by intrathoracic inoculation and by feeding on pledgets soaked with a virus-blood-sucrose mixture. All mosquito strains were uniformly susceptible to infection by intrathoracic inoculation, but 50% infective doses for field and colonized strains varied as much as 1000- to 1500-fold, respectively, by pledget feeding. Dose-response curves obtained by pledget feeding revealed that some field and laboratory strains of C. tarsalis were quite heterogeneous for susceptibility since some individual females could be infected after ingestion of small concentrations of virus and others could not be infected by increasing the concentration of virus by 1000- to 10,000-fold. Variability in viral susceptibility of different mosquito strains could not be correlated with differences in autogeny rates or organophosphorus insecticide resistance.