Stress-induced HSP70 from Musca domestica plays a functionally significant role in the immune system.
Stress-induced HSP70 from Musca domestica plays a functionally significant role in the immune system.
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DOI：
10.1016/j.jinsphys.2012.06.007
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发表时间：
2012-09
影响因子：
2.2
通讯作者：
T. Tang;Chen Wu;Jigang Li;Guodong Ren;Dawei Huang;Fengsong Liu
中科院分区：
文献类型：
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作者：
T. Tang;Chen Wu;Jigang Li;Guodong Ren;Dawei Huang;Fengsong Liu
70kDa热休克蛋白（HSP 70）家族成员作为重要的分子伴侣，在生物体的应激耐受和天然免疫中发挥重要作用。从家蝇中分离到一种新的诱导型热休克蛋白70（MdHSP 70）的全长cDNA。该cDNA全长2411 bp，开放阅读框为1956 bp，编码651个氨基酸。利用实时定量聚合酶链反应（qPCR），我们研究了热休克，镉胁迫和细菌的反应基因的转录谱。在热休克和镉胁迫下，MdHSP 70的表达增加。大肠杆菌或金黄色葡萄球菌刺激可显著诱导MdHSP 70的表达。向幼虫饲喂表达靶向MdHSP 70基因的dsRNA的细菌。我们的研究结果表明，在热休克，镉胁迫和细菌入侵的MdHSP 70的dsRNA处理的幼虫的高死亡率，表明MdHSP 70是潜在的参与应激和免疫反应的家蝇，可能有助于保护免受细胞损伤。
As important molecular chaperones, members of the 70kDa heat shock protein (HSP70) family play essential roles in stress tolerance and innate immunity in organisms. The full-length complementary DNA (cDNA) of a novel inducible HSP70, named as MdHSP70, was isolated from Musca domestica. The cDNA clone consisted of 2411 bp with a 1956 bp open reading frame which encodes 651 amino acids. Using real-time quantitative polymerase chain reaction (qPCR), we investigated the transcriptional profile of the gene under heat shock, cadmium stress and in response to bacteria. Increased expression of MdHSP70 was observed in response to both heat shock and Cd stress. The expression of MdHSP70 was significantly induced by Escherichia coli or Staphylococcus aureus stimulation. Larvae were fed bacteria expressing dsRNA targeting the MdHSP70 gene. Our results showed high mortality in larvae treated with dsRNA of MdHSP70 at heat shock, Cd stress and bacterial invasion, suggesting that MdHSP70 is potentially involved in the stress and immune responses of the house fly and perhaps contributes to protection against cellular injury.