Nanocomposite hydrogels for cartilage tissue engineering: mesoporous silica nanofibers interlinked with siloxane derived polysaccharide
Nanocomposite hydrogels for cartilage tissue engineering: mesoporous silica nanofibers interlinked with siloxane derived polysaccharide
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DOI：
10.1007/s10856-013-4951-0
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发表时间：
2013-08-01
影响因子：
3.7
通讯作者：
Le Bideau, Jean
中科院分区：
文献类型：
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作者：
Buchtova, Nela;Rethore, Gildas;Le Bideau, Jean
对软骨微创手术的注射材料进行了合成和深入研究。这些混合材料的概念是基于提供足够高的机械性能沿着良好的软骨细胞增殖培养基。本文提出的不寻常的纳米复合材料水凝胶是基于与介孔二氧化硅纳米纤维互连的硅氧烷衍生的羟丙基甲基纤维素（Si-HPMC）。通过荧光方法检查纳米复合材料的强制性均匀性，这表明二氧化硅纳米纤维分散体被实现到纳米尺度，表明二氧化硅纳米纤维有效地固定到Si-HPMC支架上。由于亲水性二氧化硅纳米纤维和Si-HPMC链的侧链硅烷醇盐基团之间的化学亲和力，实现了这种分散和固定。调节纳米电荷的量允许调节这些可注射的生物相容性混合水凝胶的所得机械特征。在高达3wt%的二氧化硅纳米纤维的纳米复合物水凝胶内检查hASC干细胞和SW 1353软骨细胞的活力。
Injectable materials for mini-invasive surgery of cartilage are synthesized and thoroughly studied. The concept of these hybrid materials is based on providing high enough mechanical performances along with a good medium for chondrocytes proliferation. The unusual nanocomposite hydrogels presented herein are based on siloxane derived hydroxypropylmethylcellulose (Si-HPMC) interlinked with mesoporous silica nanofibers. The mandatory homogeneity of the nanocomposites is checked by fluorescent methods, which show that the silica nanofibres dispersion is realized down to nanometric scale, suggesting an efficient immobilization of the silica nanofibres onto the Si-HPMC scaffold. Such dispersion and immobilization are reached thanks to the chemical affinity between the hydrophilic silica nanofibers and the pendant silanolate groups of the Si-HPMC chains. Tuning the amount of nanocharges allows tuning the resulting mechanical features of these injectable biocompatible hybrid hydrogels. hASC stem cells and SW1353 chondrocytic cells viability is checked within the nanocomposite hydrogels up to 3 wt% of silica nanofibers.