Computational identification of plant MicroRNAs and their targets, including a stress-induced miRNA
Computational identification of plant MicroRNAs and their targets, including a stress-induced miRNA
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DOI：
10.1016/j.molcel.2004.05.027
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发表时间：
2004-06-18
期刊：
影响因子：
16
通讯作者：
Bartel, DP
中科院分区：
文献类型：
--
作者：
Jones-Rhoades, MW;Bartel, DP
MicroRNAs(MiRNAs)类似于21个核苷酸的RNA，其中一些已被证明在植物发育过程中发挥着重要的遗传调控作用。我们发展了比较基因组学方法来系统地鉴定在拟南芥和水稻中保守的miRNAs及其靶标。23个miRNA候选基因代表了7个新发现的基因家族，在拟南芥中得到了实验验证，使已报道的miRNA基因总数达到92个，代表22个家族。通过检测诊断植物中miRNA切割的mRNA片段，确认了19个新鉴定的目标候选基因。总体而言，植物miRNAs有很强的针对控制发育的基因的倾向，特别是那些转录因子和F-box蛋白的基因。然而，植物miRNAs具有超越发育的保守调节功能，因为它们也针对超氧化物歧化酶、漆酶和ATP硫酰基酶。硫酸盐饥饿后，硫酸酯酶靶向的miR395的表达增加，表明环境胁迫可以诱导miRNAs的表达。
MicroRNAs (miRNAs) are similar to21-nucleotide RNAs, some of which have been shown to play important generegulatory roles during plant development. We developed comparative genomic approaches to systematically identify both miRNAs and their targets that are conserved in Arabidopsis thaliana and rice (Oryza sativa). Twenty-three miRNA candidates, representing seven newly identified gene families, were experimentally validated in Arabidopsis, bringing the total number of reported miRNA genes to 92, representing 22 families. Nineteen newly identified target candidates were confirmed by detecting mRNA fragments diagnostic of miRNA-directed cleavage in plants. Overall, plant miRNAs have a strong propensity to target genes controlling development, particularly those of transcription factors and F-box proteins. However, plant miRNAs have conserved regulatory functions extending beyond development, in that they also target superoxide dismutases, laccases, and ATP sulfurylases. The expression of miR395, the sulfurylase-targeting miRNA, increases upon sulfate starvation, showing that miRNAs can be induced by environmental stress.