A review of solute encapsulating nanoparticles used as delivery systems with emphasis on branched amphipathic peptide capsules.
A review of solute encapsulating nanoparticles used as delivery systems with emphasis on branched amphipathic peptide capsules.
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DOI：
10.1016/j.abb.2016.02.027
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发表时间：
2016-04-15
影响因子：
3.9
通讯作者：
Tomich JM
中科院分区：
文献类型：
--
作者：
Barros SM;Whitaker SK;Sukthankar P;Avila LA;Gudlur S;Warner M;Beltrão EI;Tomich JM
正在开发各种策略来改善药物的递送和增加药物的生物半衰期。为了解决这些问题，药物输送技术依赖于不同的纳米级分子，包括：脂质囊泡、病毒衣壳和纳米颗粒。多肽是许多纳米材料的组成部分，并且克服了与脂质或病毒传递系统相关的一些限制，例如可调性、稳定性、特异性、炎症性和抗原性。本文综述了自组装形成囊泡/胶囊的生物基药物递送纳米材料的发展。而脂质囊泡在这些结构中最为突出；基于肽的结构正在出现，特别是肽双层分隔胶囊。新型生物材料支链两亲肽胶囊（BAPCs）显示出许多令人满意的性能。这些纳米球由两个支链肽组成- bis(FLIVI)- k - kkkk和bis(FLIVIGSII)- k - kkkk，其分子结构类似于二酰基磷酸甘油酯。它们经过超分子自组装，形成溶剂填充的双层圈定胶囊，大小从20纳米到2微米不等，具体取决于退火温度和时间。它们能够封装不同的荧光染料、治疗药物、放射性核素甚至小蛋白质。虽然与脂质囊泡具有许多相同的特性，但bapc更加健壮。他们已经分析了稳定性，大小，细胞摄取和定位，细胞内保留和生物分布在培养和体内。
Various strategies are being developed to improve delivery and increase the biological half-lives of pharmacological agents. To address these issues, drug delivery technologies rely on different nano-sized molecules including: lipid vesicles, viral capsids and nano-particles. Peptides are a constituent of many of these nanomaterials and overcome some limitations associated with lipid-based or viral delivery systems, such as tune-ability, stability, specificity, inflammation, and antigenicity. This review focuses on the evolution of bio-based drug delivery nanomaterials that self-assemble forming vesicles/capsules. While lipid vesicles are preeminent among the structures; peptide-based constructs are emerging, in particular peptide bilayer delimited capsules. The novel biomaterial— Branched Amphiphilic Peptide Capsules (BAPCs) display many desirable properties. These nano-spheres are comprised of two branched peptides— bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK, designed to mimic diacyl-phosphoglycerides in molecular architecture. They undergo supramolecular self-assembly and form solvent-filled, bilayer delineated capsules with sizes ranging from 20 nm to 2 microns depending on annealing temperatures and time. They are able to encapsulate different fluorescent dyes, therapeutic drugs, radionuclides and even small proteins. While sharing many properties with lipid vesicles, the BAPCs are much more robust. They have been analyzed for stability, size, cellular uptake and localization, intra-cellular retention and, bio-distribution both in culture and in vivo.