Using lipid analysis and hyphal length to quantify AM and saprotrophic fungal abundance along a soil chronosequence
Using lipid analysis and hyphal length to quantify AM and saprotrophic fungal abundance along a soil chronosequence
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DOI：
10.1016/j.soilbio.2004.08.019
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发表时间：
2005-03-01
影响因子：
9.7
通讯作者：
Ekenler, M
中科院分区：
文献类型：
--
作者：
Balser, TC;Treseder, KK;Ekenler, M
我们评估使用的签名脂肪酸和直接菌丝计数作为工具来检测和量化丛枝菌根（AM）和腐养真菌（SF）生物量在夏威夷土壤沿着自然土壤肥力梯度。磷脂16：1omega5c和18：2omega6，9c分别被用作AM和腐解真菌生物量的指标。磷脂分析和菌丝长度表明，AMF的生物量最大的最高肥力的网站，和最低的磷限制植物生长。腐解真菌生物量没有变化。菌丝长度计数似乎低估了SF丰度，而磷脂AMF：SF比例符合预期。本研究表明，磷脂可能是一个有价值的和可靠的工具，研究土壤中AM和腐解真菌的丰度，分布和相互作用。(C)2004爱思唯尔有限公司保留所有权利。
We evaluate the use of signature fatty acids and direct hyphal counts as tools to detect and quantify arbuscular mycorrhizal (AM) and saprotrophic fungal (SF) biomass in three Hawaiian soils along a natural soil fertility gradient. Phospholipids16:1omega5c and 18:2omega6,9c were used as an index of AM and saprotrophic fungal biomass, respectively. Both phospholipid analysis and hyphal length indicated that the biomass of AMF was greatest at the highest fertility site, and lowest where phosphorus limits plant growth. Saprotrophic fungal biomass did not vary. Hyphal length counts appeared to under-estimate SF abundance, while the phospholipid AMF:SF ratio was in line with expectations. This study indicates that phospholipids may be a valuable and reliable tool for studying the abundance, distribution, and interactions between AM and saprotrophic fungi in soil. (C) 2004 Elsevier Ltd. All rights reserved.