Overexpression of multiple cytochrome P450 genes associated with sulfoxaflor resistance in Aphis gossypii Glover
Overexpression of multiple cytochrome P450 genes associated with sulfoxaflor resistance in Aphis gossypii Glover
复制标题
棉蚜中与氟啶虫胺抗性相关的多个细胞色素 P450 基因的过表达
DOI：
10.1016/j.pestbp.2019.03.021
复制
发表时间：
2019-06-01
影响因子：
4.7
通讯作者：
Gao, Xiwu
中科院分区：
文献类型：
--
作者：
Ma, Kangsheng;Tang, Qiuling;Gao, Xiwu
氟啶虫胺磺酰亚胺是第一种商品化的杀虫药剂，对多种植食性害虫具有很好的防治效果，在我国已作为替代杀虫剂应用于棉蚜的防治。本研究旨在探讨棉蚜对氟啶虫胺磺草胺的抗性风险、交互抗性模式及潜在抗性机制。A.用氟啶虫胺对新疆棉株进行连续选择，获得抗性倍数为245倍的新品系。SulR菌株对吡虫啉（80.8倍）、啶虫脒（19.3倍）、噻虫嗪（10.0倍）和氟啶虫脲（107.5倍）产生了交叉抗性，而对马拉硫磷、氧化乐果、联苯菊酯、灭多威和丁硫克百威没有检测到交叉抗性。丁醚双壬酯和S，S，S-三丁基三硫代磷酸酯可使氟啶虫胺腈对SulR菌株的毒力分别增加5.99倍和4.18倍，而与马来酸二乙酯无协同作用。SulR菌株的P450 s和羧酸酯酶活性明显高于SS菌株。进一步的基因表达测定表明，SulR菌株中有9个P450基因表达量显著增加，抑制CYP 6CY 13和CYP 6CY 19基因表达显著增加SulR蚜虫对氟啶虫胺砜的敏感性。这些结果表明，细胞色素P450单加氧酶可能参与了棉蚜对氟啶虫胺腈的抗性。
Sulfoxaflor is the first commercially available sulfoximine insecticide, which exhibits highly efficacy against many sap-feeding insect pests and has been applied as an alternative insecticide against cotton aphid in China. This study was conducted to investigate the risk of resistance development, the cross-resistance pattern and the potential resistance mechanisms of sulfoxaflor in Aphis gossypii. A colony (SulR strain) of A. gossypii with 245-fold resistance, originated from Xinjiang field population, was established by continuous selection using sulfoxaflor. The SulR strain has developed cross-resistance to imidacloprid (80.8-fold), acetamiprid (19.3-fold), thiamethoxam (10.0-fold), and flupyradifurone (107.5-fold), while no cross-resistance was detected to malathion, omethoate, bifenthrin, methomyl, and carbosulfan. Piperonyl butoxide and S, S, S-tributyl phosphorotrithioate could significantly increase the toxicity of sulfoxaflor to the SulR strain by 5.99- and 4.18-fold, respectively, whereas no synergistic effect with diethyl maleate was observed. The activities of P450s and carboxylesterase were significantly higher in the SulR strain than that in the SS strain. Further gene expression determination demonstrated that nine P450 genes were significantly increased in SulR strain and suppression the expression of CYP6CY13 and CYP6CY19 by RNAi significantly increased the susceptibility of SulR adult aphids to sulfoxaflor. These results demonstrated that the enhancing detoxification by cytochrome P450 monooxygenase may be involved in A.gossypii resistance to sulfoxaflor.