KETAMINE AND PHENCYCLIDINE CAUSE A VOLTAGE-DEPENDENT BLOCK OF RESPONSES TO L-ASPARTIC ACID
KETAMINE AND PHENCYCLIDINE CAUSE A VOLTAGE-DEPENDENT BLOCK OF RESPONSES TO L-ASPARTIC ACID
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DOI：
10.1016/0304-3940(85)90414-8
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发表时间：
1985-01-01
影响因子：
2.5
通讯作者：
MACDONALD, JF
中科院分区：
文献类型：
--
作者：
HONEY, CR;MILJKOVIC, Z;MACDONALD, JF
兴奋性氨基酸通过增加膜电导来使中央哺乳动物神经元去极化。这种电导的增加可以取决于电压依赖性（即N-甲基-D-天冬氨酸或L-天冬氨酸（L-ASP））或不依赖电压的（即Kainic Acid（DA）），取决于该通道是否被阻塞由mg2+ [8，9]。细胞内记录是由分解的小鼠脊髓细胞进行的，并使用恒定电流技术计算电导。解离性麻醉药，氯胺酮和苯二肽引起了L-ASP引起的电导变化的选择性抑郁，但不是Ka。在整个细胞电压钳（在没有细胞外Mg2+的情况下），对L-ASP的反应抑制是高度依赖性的，这表明该通道会受到阻断。
Excitatory amino acids depolarize central mammalian neurons by increasing membrane conductance. This increase in conductance can be voltage-dependent (i.e. N-methyl-D-aspartate or L-aspartic acid (L-ASP)) or voltage-independent (i.e. kainic acid (DA)) depending on whether or not the channel is blocked by Mg2+ [8, 9]. Intracellular recordings were made from dissociated mouse spinal cord cells and conductance was calculated using constant current techniques. The dissociative anesthetics, ketamine and phencyclidine caused a selective depression in the change in conductance evoked by L-ASP but not that by KA. Under whole cell voltage-clamp (in the absence of extracellular Mg2+) this depression of responses to L-ASP was found to be highly voltage-dependent suggesting a blockade of the channel.