Increased interleukin-10 messenger RNA expression in atopic allergy and asthma.
Increased interleukin-10 messenger RNA expression in atopic allergy and asthma.
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特应性过敏和哮喘中白细胞介素 10 信使 RNA 表达增加。
DOI：
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发表时间：
1996
影响因子：
6.4
通讯作者：
Qutayba A. Hamid
中科院分区：
文献类型：
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作者：
D. S. Robinson;A. Tsicopoulos;Qiu Meng;Steven Durham;A. Kay;Qutayba A. Hamid
白细胞介素-10（IL-10）抑制T淋巴细胞增殖和细胞因子的产生。我们用原位杂交检测了过敏性哮喘、过敏原和结核菌素皮肤反应中IL-10信使RNA（mRNA）的表达。与对照组相比，10例有症状的哮喘患者支气管肺泡灌洗（BAL）细胞中IL-10 mRNA阳性的比例增加（17.5%对5.2%; P < 0.001）。在另一组6名轻度特应性哮喘患者中，与来自相同受试者的稀释液激发BAL相比，变应原吸入激发后24 h BAL细胞中IL-10 mRNA阳性的比例增加（24%对10%; P < 0.005）。通过同时原位杂交和免疫细胞化学，IL-10 mRNA定位于两个CD 3 + T细胞和CD 68+肺泡巨噬细胞在BAL中，与对照组相比，在有症状的哮喘患者和过敏原激发后，与轻度哮喘患者的稀释液激发相比，具有显着更突出的T细胞信号。已经表明IL-10的产生是T细胞活化后的晚期事件。为了检测IL-10 mRNA表达的动力学和特异性，在皮肤注射过敏原或结核菌素后1、6和48 h从特应性结核菌素敏感受试者中获得皮肤活检。在两种刺激下，与注射适当稀释剂的对照部位（注射后24 h活检）相比，6 h时IL-10 mRNA阳性细胞增加（变应原P < 0.01，结核菌素P < 0.02）。这些发现与哮喘患者气道巨噬细胞和T淋巴细胞均表达IL-10 mRNA以及T淋巴细胞对过敏原应答诱导IL-10 mRNA表达的假设一致。这种反应也可能发生在其他类型的细胞介导的炎症中。
Interleukin-10 (IL-10) inhibits T-lymphocyte proliferation and production of cytokines. We have examined expression of IL-10 messenger RNA (mRNA) in atopic asthma and in allergen and tuberculin skin responses by in situ hybridization. The proportion of bronchoalveolar lavage (BAL) cells positive for IL-10 mRNA was increased in a group of 10 symptomatic asthmatics when compared with control subjects (17.5% versus 5.2% BAL cells positive; P < 0.001). In a separate group of six mild atopic asthmatics, there was an increased proportion of BAL cells positive for IL-10 mRNA 24 h after allergen inhalation challenge compared with diluent challenge BAL from the same subjects (24% versus 10%; P < 0.005). By simultaneous in situ hybridization and immunocytochemistry, IL-10 mRNA was localized to both CD3+ T cells and CD68+ alveolar macrophages in BAL, with a significantly more prominent T-cell signal in the symptomatic asthmatics compared with control subjects and after allergen challenge compared with diluent challenge of the mild asthmatic subjects. It has been suggested that IL-10 production is a late event after T-cell activation. To examine kinetics and specificity of IL-10 mRNA expression, skin biopsies were obtained from atopic, tuberculin-sensitive subjects at 1, 6, and 48 h after cutaneous injection of allergen or tuberculin. With both stimuli, there was an increase in IL-10 mRNA-positive cells at 6 h when compared with control sites injected with appropriate diluent which were biopsied 24 h after injection (P < 0.01 for allergen and P < 0.02 for tuberculin). These findings are compatible with the hypothesis that IL-10 mRNA is expressed in both macrophages and T lymphocytes in the airway in asthma and that IL-10 mRNA expression is induced from T lymphocytes in response to allergen. This response may also occur in other types of cell-mediated inflammation.
IL-10 对实验性小鼠血吸虫病 T 辅助细胞反应的调节。
DOI：
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发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
FloresVillanueva,PO;Reiser,H;Stadecker,MJ
通讯作者：
Stadecker,MJ