DISTINCT FUNCTIONAL PHENOTYPES OF CLONED IA-RESTRICTED HELPER T-CELLS
DISTINCT FUNCTIONAL PHENOTYPES OF CLONED IA-RESTRICTED HELPER T-CELLS
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DOI：
10.1084/jem.162.1.188
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发表时间：
1985-01-01
影响因子：
15.3
通讯作者：
BOTTOMLY, K
中科院分区：
文献类型：
--
作者：
KIM, J;WOODS, A;BOTTOMLY, K
通过大量不同克隆的自身Ia特异性辅助T细胞（Th）克隆对鼠磷酸胆碱（PC）特异性B细胞的激活的分析，允许将这种T细胞分类为4种不同的功能类型。1型和2型诱导B细胞以抗原特异性、Ia限制性方式分泌抗PC抗体。3型细胞诱导抗原特异性、Ia限制性B细胞增殖，但不导致特异性抗体形成，并且先前已显示具有抑制功能。4型细胞是自身反应性的，并诱导抗原非依赖性B细胞活化和抗体分泌。详细分析了1型和2型Th克隆之间的区别。在批量培养中，1型克隆系产生独特异质性抗PC抗体应答，而2型克隆系诱导由T15独特型主导的较大应答。在有限稀释分析中，2型细胞诱导的T15+ PC特异性前体B细胞比1型细胞多4倍，此外，诱导的T15+ PC特异性B细胞爆发量更大。4型克隆可细分为1型样细胞和2型样细胞。在广泛的抗原和细胞剂量范围内观察到功能表型的这些差异。这些不同的功能类型的Th细胞的行为的详细分析，应允许更好地了解抗原引发的，IA限制性Th细胞的混合群体的功能特性。
Analysis of activation of murine phosphorylcholine (PC)-specific B cells by a large number of different cloned, self Ia-specific helper T cell (Th) clones has permitted the classification of such T cells into 4 distinct functional types. Types 1 and 2 induce B cells to secrete anti-PC antibody in an antigen-specific, Ia-restricted fashion. Type 3 cells induce antigen-specific, Ia-restricted B cell proliferation, but do not lead to specific antibody formation, and have been shown previously to have suppressor functions. Type 4 cells are autoreactive and induce antigen-independent B cell activation and antibody secretion. The distinction between type 1 and type 2 Th clones was analyzed in detail. In bulk cultures, type 1 cloned lines generate an idiotypically heterogenous anti-PC antibody response, whereas type 2 cloned lines induce a larger response that is dominated by the T15 idiotype. In limiting-dilution analyses, type 2 cells induce 4-fold more T15+, PC-specific precursor B cells than do type 1 cells, and in addition, induce larger burst sizes for T15+, PC-specific B cells. Type 4 clones can be subdivided into cells that are type 1-like, and cells that are type 2-like. These differences in functional phenotype are seen over a broad range of antigen and cell doses. Detailed analysis of the behavior of these distinct functional types of Th should allow a better understanding of the functional properties of mixed populations of antigen-primed, Ia-restricted Th cells.