Longitudinal study of transmission, diversity, and stability of Streptococcus mutans and Streptococcus sobrinus genotypes in Brazilian nursery children
Longitudinal study of transmission, diversity, and stability of Streptococcus mutans and Streptococcus sobrinus genotypes in Brazilian nursery children
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DOI：
10.1128/jcm.42.10.4620-4626.2004
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发表时间：
2004-10-01
影响因子：
9.4
通讯作者：
Gonçalves, RB
中科院分区：
文献类型：
--
作者：
Klein, MN;Flório, FM;Gonçalves, RB
本研究的目的是对儿童口腔中变形链球菌和远缘链球菌的定植情况进行随访评估，包括母婴垂直传播的模式、基因型多样性和菌株的稳定性。研究对象为16对母子，监测时间为20个月。每两个月收集一次儿童的唾液、舌背、牙槽嵴粘膜和牙菌斑样本。还收集了母亲的唾液样本。分离鉴定后，采用随机引物PCR方法对S.变形链球菌（968株）和S. sobrinus（111株）。在获得菌株时，这些儿童携带1至4种不同的S基因型。变形链球菌和只有一种基因型的S. sobrinus。虽然S.变形链球菌的患病率和基因型多样性均高于链球菌。sobrinus，存在匹配的S. mutans和S. sobrinus相似（分别为81.25%和83.33%的母子对），表明两个物种的垂直传播。这项纵向研究表明，S的基因型多样性增加。随访期间口腔中的变异株：大多数最初获得的基因型持续存在，通常是母亲传播的基因型，有些在随访期间丢失;还获得了新的菌株。总之，S. mutans和S.从母体或替代来源获得的sobrinus基因型可能在口腔中表现出有效的持久性和/或在儿童口腔中的短暂检测，反映了儿童口腔微生物群的持续发育。
The aim of this study was to perform a follow-up evaluation of the Streptococcus mutans and Streptococcus sobrinus colonization profile of children's oral cavities, which included the pattern of vertical transmission from mother to child, genotypic diversity, and stability of the strains. The subjects were 16 mother-child pairs, who were monitored for 20 months. Samples of saliva, tongue dorsum, alveolar ridge mucosa, and dental plaque from the children were collected bimonthly. Saliva samples from the mothers were also collected. After isolation and identification, the arbitrarily primed PCR method was performed for the genotypic characterization of S. mutans (968 isolates) and S. sobrinus (111 isolates). At the time the strains were acquired, the children harbored one to four distinct genotypes of S. mutans and only one genotype of S. sobrinus. Although S. mutans prevalence and genotypic diversity were greater than those of S. sobrinus, the presence of matching genotypes of S. mutans and S. sobrinus was similar (in 81.25 and 83.33% of mother-child pairs, respectively), suggesting vertical transmission for both species. This longitudinal study showed an increase in genotypic diversity of S. mutans in the oral cavity during the follow-up period: most of the initially acquired genotypes persisted, normally those genotypes transmitted by the mother, and some were lost during follow-up; new strains were also acquired. In conclusion, S. mutans and S. sobrinus genotypes acquired from maternal or alternative sources may show effective persistence in the oral cavity and/or transitory detection in the children's mouths, reflecting the continuous development of oral microbiota in children.