MAGNETIC LABELING AND CELL SORTING
MAGNETIC LABELING AND CELL SORTING
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DOI：
10.1016/0022-1759(84)90029-2
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发表时间：
1984-01-01
影响因子：
2.2
通讯作者：
SYKES, NL
中科院分区：
文献类型：
--
作者：
OWEN, CS;SYKES, NL
将磁铁矿-抗体缀合物的磁性标记与磁过滤（高梯度磁分离）相结合，以实现从单细胞悬浮液中快速有效地分离选定的细胞群。超过 108 个细胞的样品可在约 5 分钟内进行分级并完全恢复。该系统应用于使用红细胞（羊或鸡）和针对物种确定的细胞表面抗原的商业抗体的模型系统。观察到标记细胞的富集因子高达 37 倍。该方法对实验方案中的细节以及样品中未标记细胞的数量相对不敏感。因此，该方法易于使用，并且可以轻松扩展以处理总共包含 108 个标记细胞（总计 1011 个或更多）的大样本。它应该可用作悬浮液的预富集方案，其中感兴趣的细胞很少，因此荧光激活仪器的分选效率非常低。
Magnetic labeling by a magnetite-antibody conjugate was combined with magnetic filtration (high gradient magnetic separation) to effect a rapid and efficient separation of a selected cell population from a suspension of single cells. Samples of > 108 cells could be fractionated in about 5 min with complete recovery. The system was applied to a model system using red blood cells (sheep or chicken) and commercial antibodies against species-determined cell surface antigens. Enrichments of labeled cells by factors of up to 37-fold were observed. The approach was relatively insensitive to details in the experimental protocol and to the number of unlabeled cells which were in the sample. Thus, the method was easy to use and can readily be scaled up to handle large samples containing 108 labeled cells in a total in 1011 or more. It should be useful as a pre-enrichment scheme for suspensions in which cells of interest are rare and, consequently, very inefficiently sorted by fluorescence-activated instruments.