Distinct sets of PIWI proteins produce arbovirus and transposon-derived piRNAs in Aedes aegypti mosquito cells.
Distinct sets of PIWI proteins produce arbovirus and transposon-derived piRNAs in Aedes aegypti mosquito cells.
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DOI：
10.1093/nar/gkv590
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发表时间：
2015-07-27
影响因子：
14.9
通讯作者：
van Rij RP
中科院分区：
文献类型：
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作者：
Miesen P;Girardi E;van Rij RP
PIWI 相互作用 RNA (piRNA) 途径对于许多模型生物中的转座子沉默至关重要。其卓越的效率依赖于一种称为“乒乓循环”的复杂放大机制。在甲病毒感染的伊蚊中，具有表明乒乓球依赖性生物发生的序列特征的piRNA是由病毒RNA产生的。与其他模式生物相比，伊蚊中的 PIWI 家族得到了扩展，这提高了这些昆虫中单个 PIWI 蛋白功能多样化的可能性。在这里，我们表明 Piwi5 和 Ago3，但其他 PIWI 家族成员，对于受感染的埃及伊蚊细胞中辛德比斯病毒 RNA 的 piRNA 生物合成是必需的。相比之下，转座子生产 piRNA 依赖于一组用途更广泛的 PIWI 蛋白，其中一些蛋白对病毒 piRNA 生物合成没有贡献。这些结果表明，功能特化允许不同的蚊子 PIWI 蛋白处理来自不同内源和外源的 RNA。
The PIWI-interacting RNA (piRNA) pathway is essential for transposon silencing in many model organisms. Its remarkable efficiency relies on a sophisticated amplification mechanism known as the ping-pong loop. In Alphavirus-infected Aedes mosquitoes, piRNAs with sequence features that suggest ping-pong-dependent biogenesis are produced from viral RNA. The PIWI family in Aedes mosquitoes is expanded when compared to other model organisms, raising the possibility that individual PIWI proteins have functionally diversified in these insects. Here, we show that Piwi5 and Ago3, but none of the other PIWI family members, are essential for piRNA biogenesis from Sindbis virus RNA in infected Aedes aegypti cells. In contrast, the production of piRNAs from transposons relies on a more versatile set of PIWI proteins, some of which do not contribute to viral piRNA biogenesis. These results indicate that functional specialization allows distinct mosquito PIWI proteins to process RNA from different endogenous and exogenous sources.