Involvement of GnRH neuron in the spermatogonial proliferation of the scallop, Patinopecten yessoensiss
Involvement of GnRH neuron in the spermatogonial proliferation of the scallop, Patinopecten yessoensiss
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DOI：
10.1002/mrd.20544
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发表时间：
2007-01-01
影响因子：
2.5
通讯作者：
Kijima, Akihiro
中科院分区：
文献类型：
--
作者：
Nakamura, Satoshi;Osada, Makoto;Kijima, Akihiro
本研究的目的是定量分析生殖细胞的增殖模式，并通过体外实验证明神经参与扇贝精原细胞的增殖。掺入BrdU的免疫细胞化学用于鉴定有丝分裂活性的生殖腺细胞。生殖细胞的增殖模式分为两个阶段：第一阶段，卵原细胞和精原细胞缓慢增殖，通过生长阶段：第二阶段，卵原细胞发育成卵母细胞和精原细胞开始迅速增殖，从成熟阶段通过产卵阶段。抗哺乳动物（m）GnRH抗体检测到的神经元稀疏分布在足神经节，主要分布在大脑神经节中的两种性别的生长阶段。在生长阶段收集的两性大脑和足神经节（CPG）的提取物促进睾丸组织体外培养中精原细胞的增殖以及mGnRH。而CPG提取物对卵原细胞增殖无影响。添加mGnRH拮抗剂和抗mGnRH抗体可抑制CPG和mGnRH诱导的有丝分裂活性的增加，提示CPG中的GnRH样肽对扇贝精原细胞增殖有调节作用。在血细胞裂解物中观察到与CPG提取物和mGnRH相同的有丝分裂活性，但在血清中未观察到。这些结果表明，扇贝精原细胞的II期增殖可能受CPG中GnRH神经元的神经内分泌控制。
The aim of this study was to quantitatively analyze a pattern of proliferation of gonial cells and to demonstrate neural involvement in spermatogonial proliferation of the scallop by the in vitro experiment. Immunocytochemistry for incorporated BrdU was used to identify mitotically active gonial cells. The pattern of proliferation of gonial cells was divided into two phases: phase I; oogonia and spermatogonia slowly proliferate through the growing stage: phase II; oogonia develop into oocytes and spermatogonia start to proliferate rapidly from the mature stage through the spawning stage. The neurons detected with anti-mammalian (m)GnRH antibody were distributed sparsely in the pedal ganglion and predominantly in the cerebral ganglion of both sexes at the growing stage. The extracts from the cerebral and pedal ganglion (CPG) of both sexes collected at the growing stage promoted proliferation of spermatogonia in the in vitro culture of the testicular tissue as well as mGnRH. However, CPG extract had no effect on oogonial proliferation. The increased mitotic activity induced by CPG and mGnRH was abolished by the addition of mGnRH antagonists and anti-mGnRH antibody, suggesting that the spermatogonial proliferation is regulated by GnRH-like peptide in CPG of the scallop. The same mitotic activity as CPG extract and mGnRH was observed in the hemocyte lysate, but not in the serum. These findings suggest that the spermatogonial proliferation at phase II in the scallop may be under the neuroendocrine control by GnRH neuron in CPG.