A highly sensitive SPRi biosensing strategy for simultaneous detection of multiplex miRNAs based on strand displacement amplification and AuNP signal enhancement
A highly sensitive SPRi biosensing strategy for simultaneous detection of multiplex miRNAs based on strand displacement amplification and AuNP signal enhancement
复制标题
基于链置换扩增和 AuNP 信号增强的高灵敏度 SPRi 生物传感策略，用于同时检测多重 miRNA。
作者：
Xun Min
在此，双通道表面等离子体共振成像（SPRi）生物传感器已开发用于同时和高灵敏度检测的多重miRNA的基础上链置换扩增（SDA）和DNA功能化的金纳米粒子信号增强。在靶miRNA（miR-21或miR-192）的存在下，miRNA可以与相应的发夹探针（H）特异性杂交并启动SDA，导致大量触发物。随后，释放的触发物的两个部分可以与捕获探针（CP）和DNA功能化的金纳米颗粒杂交，在芯片表面组装具有大质量的DNA三明治。实现了显着放大的SPR信号读出。这种建立的生物传感方法能够同时检测多重miRNA，其中miR-21的检测限低至0.15 pM，miR-192的检测限低至0.22 pM。该方法具有良好的专属性和可接受的重现性。此外，所开发的方法被应用于在复杂的矩阵中的目标miRNA的测定。因此，这种开发的SPRi生物传感方法可能为生物医学研究和临床诊断中的miRNA检测提供潜在的替代工具。
Herein, a dual channel surface plasmon resonance imaging (SPRi) biosensor has been developed for the simultaneous and highly sensitive detection of multiplex miRNAs based on strand displacement amplification (SDA) and DNA-functionalized AuNP signal enhancement. In the presence of target miRNAs (miR-21 or miR-192), the miRNAs could specifically hybridize with the corresponding hairpin probes (H) and initiate the SDA, resulting in massive triggers. Subsequently, the two parts of the released triggers could hybridize with capture probes (CP) and DNA-functionalized AuNPs, assembling DNA sandwiches with great mass on the chip surface. A significantly amplified SPR signal readout was achieved. This established biosensing method was capable of simultaneously detecting multiplex miRNAs with a limit of detection down to 0.15 pM for miR-21 and 0.22 pM for miR-192. This method exhibited good specificity and acceptable reproducibility. Moreover, the developed method was applied to the determination of target miRNAs in a complex matrix. Thus, this developed SPRi biosensing method may present a potential alternative tool for miRNA detection in biomedical research and clinical diagnosis.