Clinical grade allogeneic human mesenchymal stem cells restore alveolar fluid clearance in human lungs rejected for transplantation
Clinical grade allogeneic human mesenchymal stem cells restore alveolar fluid clearance in human lungs rejected for transplantation
复制标题
DOI：
10.1152/ajplung.00358.2013
复制
发表时间：
2014-05-01
影响因子：
4.9
通讯作者：
Lee, J. W.
中科院分区：
文献类型：
--
作者：
McAuley, D. F.;Curley, G. F.;Lee, J. W.
在肺移植中，由于潜在供体肺的利用率低，导致所有实体器官移植项目缺乏合适的供体，这主要是由于供体肺损伤和功能障碍，包括肺水肿。目前的研究旨在确定静脉注射临床级人间充质干（基质）细胞（hMSCs）是否能有效恢复人离体肺灌注模型中的肺泡液体清除率（AFC），使用的肺被认为不适合移植并经历了长时间的缺血。人肺用平衡电解质溶液中的5%白蛋白灌注，并用持续气道正压通气进行氧合。在对照叶中测量基线AFC，如果AFC受损（定义为
The lack of suitable donors for all solid-organ transplant programs is exacerbated in lung transplantation by the low utilization of potential donor lungs, due primarily to donor lung injury and dysfunction, including pulmonary edema. The current studies were designed to determine if intravenous clinical-grade human mesenchymal stem (stromal) cells (hMSCs) would be effective in restoring alveolar fluid clearance (AFC) in the human ex vivo lung perfusion model, using lungs that had been deemed unsuitable for transplantation and had been subjected to prolonged ischemic time. The human lungs were perfused with 5% albumin in a balanced electrolyte solution and oxygenated with continuous positive airway pressure. Baseline AFC was measured in the control lobe and if AFC was impaired (defined as