Freeze-fracture investigation of the red pulp of human spleen.
Freeze-fracture investigation of the red pulp of human spleen.
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DOI：
10.1007/bf02890056
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Banks, P
中科院分区：
文献类型：
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作者：
Kaiserling, E;Wolburg, H;Banks, P
这项研究关注冷冻断裂复制品中的人类脾脏的形态，并将其与超薄切片的结果进行比较。研究的材料包括胃切除术中切除的两个脾脏和因毛细胞白血病病例中脾肿大而切除的一个脾脏。当前有关脾脏超微结构的概念已普遍通过冷冻骨折技术得到证实。正如预期的那样，窦壁由紧密贴合的内皮细胞组成，在冷冻骨折复制品中可以通过细胞膜上的许多小凹来识别。与文献中所主张的观点相反，偶尔发现窦内皮细胞通过桥粒或粘附斑连接。与超薄切片中桥粒的发现相对应，在冷冻骨折复制品中观察到膜内颗粒的局灶性聚集，并且在光镜水平上发现鼻窦中桥粒斑蛋白的阳性免疫组织化学反应。文献中普遍认为窦内皮细胞可以表现出紧密连接的观点并未得到证实。然而，在血管内皮细胞之间发现了这种连接。鼻窦的环纤维通过收缩纤维与鼻窦内皮细胞紧密相连，显然具有多种功能。首先，它们有助于维持机械稳定性。其次，它们代表基底膜，窦内皮细胞与其周围环境之间通过基底膜进行交换。这可以通过这里经常发现的大量和集中收集的小窝和囊泡来表明。毛细胞在冷冻断裂复制品中没有表现出任何特征来表明与叉指网状细胞的细胞遗传学关系。
This study concerns the morphology of the human spleen in freeze-fracture replicas and compares this with the findings in ultrathin sections. The material investigated consisted of two spleens resected at gastrectomy and one resected because of splenomegaly in a case of hairy cell leukaemia. The current concepts concerning the ultrastructure of the spleen were generally confirmed with the freeze-fracture technique. The sinus walls were found, as expected, to consist of closely fitting endothelial cells, which were identifiable in freezefracture replicas by numerous caveolae of the cell membrane. Contrary to the opinion upheld in the literature, the sinus endothelial cells were occasionally found to be connected by desmosomes or maculae adhaerentes. Corresponding to the finding of desmosomes in ultrathin sections, focal collections of intramembranous particles were observed in freeze-fracture replicas and a positive immunohistochemical reaction for desmoplakin in the sinuses was found at the light microscopic level. The view generally held in the literature that sinus endothelial cells can exhibit tight junctions was not confirmed. However, such junctions were found between vascular endothelial cells. The ring fibres of the sinuses, which are closely connected to the sinus endothelial cells through contractile fibres, apparently have various functions. Firstly, they contribute towards maintaining mechanical stability. Secondly, they represent basement membranes through which exchange occurs between the sinus endothelial cells and their surroundings. This is indicated by the caveolae and vesicles that are often found here in large numbers and in focal collections. Hairy cells exhibit no features in freeze-fracture replicas to suggest a cytogenetic relationship to interdigitating reticulum cells.