Chloroplast assemblage by mechanical stimulation and its intercellular transmission in diatom cells
Chloroplast assemblage by mechanical stimulation and its intercellular transmission in diatom cells
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DOI：
10.1007/bf01279887
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发表时间：
1997-01-01
期刊：
影响因子：
2.9
通讯作者：
ShihiraIshikawa, I
中科院分区：
文献类型：
--
作者：
Makita, N;ShihiraIshikawa, I
通过接触光滑侧链藻（一种中心硅藻）的细胞，皮层细胞质中的叶绿体立即迁移到位于细胞中心的核细胞质中。迁移所需的时间约为10 s，恢复所需的时间约为15 min。诱导叶绿体组装需要7V脉冲1 ms。为了在50%的细胞中获得叶绿体聚集，所需的时间和电压成反比。高浓度的KCl也能诱导叶绿体的聚集。在无Ca ~（2+）的培养基中，接触和电刺激均不能诱导叶绿体的组装。钙通道阻断剂维拉帕米（Verapamil）在培养基中抑制叶绿体的组装。Ca ~（2+）离子载体引起叶绿体的组装。这些结果表明，质膜的去极化是诱导叶绿体组装的触发器，Ca ~（2+）通过该通道内流到细胞质是诱导叶绿体组装的必要条件。叶绿体组合被连续地传递到丝状菌落的其他细胞，尽管这些细胞仅由粘液物质连接。此外，传播发生在单独放置的牢房中。这种跳跃传播的最大可能距离是200 μ m。为了寻找任何化学递质的释放，将接触刺激施加于丝状菌落中间的一个细胞，所述丝状菌落置于沿着丝轴流动的水中。下游细胞比上游细胞更易诱导叶绿体的组装。这一结果表明，存在某种物质介导的刺激的传输。
By touching a cell of Pleurosira laevis, a centric diatom, the chloroplasts in the cortical cytoplasm immediately migrate to the nuclear cytoplasm which is located at the center of the cell. The time required for migration, is about 10 s and that for restoration is about 15 min. The touch could be replaced by an electric stimulation. A 7 V pulse for 1 ms was required for the induction of chloroplast assemblage. To obtain chloroplast assemblage in 50% of the cells, the amount of time and voltage required were inversely proportional. Higher concentrations of KCl also induced chloroplast assemblage. Neither contact nor electric stimulation in Ca2+ free medium induced chloroplast assemblage. Verapamil, a Ca2+ channel blocker, in medium suppressed the chloroplast assemblage. Ca2+ ionophore caused the chloroplast assemblage. These results suggested that the depolarization of the plasma membrane was a trigger of the induction of chloroplast assemblage and the influx of Ca2+ to the cytoplasm through the channel was essential for this. The chloroplast assemblage was transmitted successively to the other cells of the filamentous colony, though these cells were linked only by a mucilaginous substance. Furthermore, the transmission occurred in cells which were placed separately. The maximum distance possible for this jumping transmission was 200 mu m in the culture medium. To search for the release of any chemical transmitter, contact stimulation was applied to one cell at the middle of a filamentous colony which was placed in water streaming along the axis of the filament. Chloroplast assemblage was induced more in downstream cells than in upstream ones. This result suggested the existence of some substance which mediated the transmission of the stimulation.