Activated Oxytocin Neurons in the PVN-DVC Pathway in Asthmatic Rats
Activated Oxytocin Neurons in the PVN-DVC Pathway in Asthmatic Rats
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哮喘大鼠 PVN-DVC 通路中激活的催产素神经元
DOI：
10.3389/fnana.2020.00047
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发表时间：
2020-07-31
影响因子：
2.9
通讯作者：
Dong, Rong
中科院分区：
文献类型：
--
作者：
Chen, Zhe;Long, Li;Dong, Rong
哮喘是一种异质性疾病，中枢神经系统也参与了哮喘的发病过程。我们曾报道杏仁核可能通过投射到下丘脑室旁核（PVN）来调节哮喘发作。迷走神经背侧复合体（DVC）是调节呼吸的关键区域。本研究旨在观察哮喘大鼠室旁核（PVN）和DVC的活性及其相互关系。免疫组化法观察Fos和催产素（OT）的表达变化。采用小麦胚凝集素-辣根过氧化物酶（WGA-HRP）逆行追踪和OT、Fos免疫组化双重染色方法，观察HRP/OT/Fos阳性神经元在室旁核的分布。结果表明，哮喘发作时，室旁核和DVC内Fos阳性神经元随时间增加。室旁核OT阳性神经元的表达与c-Fos阳性神经元的表达呈平行趋势。HRP逆行标记神经元密集分布于室旁核的内侧亚核和外侧亚核。OT+/HRP+和Fos+/OT+/HRP+分别占HRP阳性神经元的18.14%和2.37%。本研究显示哮喘发作时室旁核和DVC被激活，室旁核内OT阳性神经元表达随时间增加。PVN与DVC之间存在联系，提示PVN内OT神经元可能投射到DVC，参与哮喘的发病。
Asthma is a heterogeneous disease, and the central nervous system (CNS) also participates in the pathogenesis of asthma. We previously reported the amygdala might regulate asthmatic attacks via projecting to the paraventricular hypothalamic nucleus (PVN). The dorsal vagal complex (DVC) is a crucial region that modulates respiratory. This study aimed to observe the activity in both PVN and DVC and the connection between PVN and DVC in asthmatic rats. Immunohistochemistry was conducted to observe the changes in Fos and oxytocin (OT) expression. Retrograde tracing using wheat germ agglutinin-horseradish peroxidase (WGA-HRP) and double immunohistochemistry for OT and Fos was used to observe the HRP/OT/Fos positive neurons distribution in the PVN. The results showed that during an asthma attack, the Fos positive neurons increased in both PVN and DVC over time. The expression of OT positive neurons in PVN showed a similar trend in parallel to the c-Fos positive neurons in PVN. The HRP retrograde-labeled neurons were densely distributed in the medial and lateral subnucleus in the PVN. OT+/HRP+ and Fos+/OT+/HRP+ accounted for 18.14%, and 2.37% of HRP-labeled neurons, respectively. Our study showed PVN and DVC were activated and the expression of OT positive neurons in PVN were increased over time during an asthma attack. The existence of connection between PVN and DVC suggested the OT neurons in PVN might project to DVC which might be involved in the pathogenesis of asthma.