Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene.
Respiratory Syncytial Virus whole-genome sequencing identifies convergent evolution of sequence duplication in the C-terminus of the G gene.
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DOI：
10.1038/srep26311
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发表时间：
2016-05-23
影响因子：
4.6
通讯作者：
Das SR
中科院分区：
文献类型：
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作者：
Schobel SA;Stucker KM;Moore ML;Anderson LJ;Larkin EK;Shankar J;Bera J;Puri V;Shilts MH;Rosas-Salazar C;Halpin RA;Fedorova N;Shrivastava S;Stockwell TB;Peebles RS;Hartert TV;Das SR
呼吸道合胞病毒（RSV）是全球范围内造成相当大的发病率和死亡率的原因，是婴儿最重要的呼吸道病毒病原体。RSV A组和B组病毒内部和之间的广泛序列变异性以及RSV的多个进化枝和亚进化枝共循环的能力可能是导致群体免疫逃避的机制。RSV的监测和大规模全基因组测序目前是有限的，但将有助于确定其进化动力学和选择性免疫逃避的网站。在这项研究中，我们对2012-2014年RSV季节期间来自田纳西州中部婴儿的92株RSV分离株进行了全基因组下一代测序。我们从A组和B组中鉴定了RSV的多个共循环分支。每个进化枝由特征性N-和0-连接的糖基化模式定义。特异性RSV基因的分析揭示了附着（G）基因的高阳性选择率。我们确定了RSV-A病毒在流通和没有最近报道的72个核苷酸的G基因序列重复。此外，我们显示了G基因序列重复和固定随时间推移的趋同进化的证据，这表明RSV与G序列重复的潜在适应性优势。
Respiratory Syncytial Virus (RSV) is responsible for considerable morbidity and mortality worldwide and is the most important respiratory viral pathogen in infants. Extensive sequence variability within and between RSV group A and B viruses and the ability of multiple clades and sub-clades of RSV to co-circulate are likely mechanisms contributing to the evasion of herd immunity. Surveillance and large-scale whole-genome sequencing of RSV is currently limited but would help identify its evolutionary dynamics and sites of selective immune evasion. In this study, we performed complete-genome next-generation sequencing of 92 RSV isolates from infants in central Tennessee during the 2012–2014 RSV seasons. We identified multiple co-circulating clades of RSV from both the A and B groups. Each clade is defined by signature N- and O-linked glycosylation patterns. Analyses of specific RSV genes revealed high rates of positive selection in the attachment (G) gene. We identified RSV-A viruses in circulation with and without a recently reported 72-nucleotide G gene sequence duplication. Furthermore, we show evidence of convergent evolution of G gene sequence duplication and fixation over time, which suggests a potential fitness advantage of RSV with the G sequence duplication.