SEARCHING FOR AUTOLYSIN FUNCTIONS - CHARACTERIZATION OF A PNEUMOCOCCAL MUTANT DELETED IN THE LYTA GENE
SEARCHING FOR AUTOLYSIN FUNCTIONS - CHARACTERIZATION OF A PNEUMOCOCCAL MUTANT DELETED IN THE LYTA GENE
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DOI：
10.1111/j.1432-1033.1986.tb09749.x
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发表时间：
1986-07-15
期刊：
影响因子：
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通讯作者：
GARCIA, E
中科院分区：
文献类型：
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作者：
SANCHEZPUELLES, JM;RONDA, C;GARCIA, E
肺炎链球菌的第一个突变体显示在编码N-乙酰胞壁酰-L-丙氨酸酰胺酶的lytA基因中的完全缺失已经被分离和表征。这种酰胺酶是以前在该物种中检测到的唯一自溶素。该突变体显示出正常的生长速率，并且可以使用染色体或质粒DNA进行转化。酰胺酶缺乏的最显著的生物学后果是形成小链（6至8个细胞）和在生长的稳定期不存在裂解。此外，该突变体表现出对β-内酰胺类抗生素
The first mutant of Streptococcus pneumoniae showing a complete deletion in the lytA gene coding for the N-acetylmuramyl-L-alanine amidase has been isolated and characterized. This amidase was previously the only autolysin detected in this species. This mutant shows a normal growth rate and can be transformed using either chromosomal or plasmid DNA. The most remarkable biological consequences of the absence of the amidase are the formation of small chains (six to eight cells) and the absence of lysis in the stationary phase of growth. In addition, this mutant exhibits a tolerant response against the .beta.-lactam antibiotics.