Analysis of bladder related nerve cuff electrode recordings from preganglionic pelvic nerve and sacral roots in pigs
Analysis of bladder related nerve cuff electrode recordings from preganglionic pelvic nerve and sacral roots in pigs
复制标题
DOI：
10.1016/s0022-5347(05)67769-5
复制
发表时间：
2000-04-01
影响因子：
6.6
通讯作者：
Sinkjær, T
中科院分区：
文献类型：
--
作者：
Jezernik, S;Wen, JG;Sinkjær, T
目的：适当的下尿路（LUT）神经的电刺激可用于膀胱功能障碍，以实现抑制和消除过度反射性逼尿肌收缩。它也可以用于随后排空膀胱。为了控制所描述的功能阶段的时间过程，需要膀胱感觉信息的知识。我们调查，如果后者可以提取的LUT神经activity.Materials和方法：在急性实验中使用10头猪，三极袖电极放置单侧周围的盆神经和S3和S2根。袖带电极信号，填充率和膀胱和直肠的压力被记录在缓慢和快速膀胱fillings/emptyings.Results：两头猪被排除在分析之外，因为没有观察到的变化，在一只动物的神经信号，因为在其他动物的电噪声问题。膀胱压力快速增加导致7只猪中有6只的骨盆神经信号突然升高（S3神经信号为6只中的3只）。对于骨盆和S3袖带信号，仅在8头猪中的3头和7头猪中的3头中记录的神经活动中反映了缓慢的膀胱压力增加。在2只动物中，骨盆神经信号清楚地反映了小的自发性膀胱收缩（仅在3只猪中观察到收缩）。除1头猪外，在S2根部未记录到慢/快膀胱充盈反应。结论：袖状电极可用于记录猪盆神经和骶神经S3根的膀胱传入信息。盆神经记录比骶神经根记录更具选择性。神经活动的增加更明显和可重复的快速膀胱压力的变化和小的自发性膀胱收缩比在缓慢的膀胱充盈。
Purpose: Electrical stimulation of appropriate lower urinary tract (LUT) nerves may be used in bladder dysfunction to achieve continence and abolish hyper-reflexic detrusor contractions. It can also be used for consequent emptying of the bladder. To control the time course of the described functional phases, knowledge of bladder sensory information is needed. We investigated if the latter could be extracted from the LUT nerve activity.Materials and Methods: In acute experiments using 10 pigs, tripolar cuff electrodes were placed unilaterally around the pelvic nerve and the S3 and S2 roots. The cuff electrode signals, filling rate and the bladder and rectal pressures were recorded during slow and fast bladder fillings/emptyings.Results: Two pigs were excluded from the analysis because of no observed changes in the nerve signals in one animal, and because of electrical noise problems in the other animal. Fast bladder pressure increases resulted in a sudden pelvic nerve signal rise in 6 out of 7 pigs (3 out of 6 for the S3 nerve signal). Slow bladder pressure increase was reflected in the recorded nerve activity only in 3 out of 8 and in 3 out of 7 pigs for the pelvic and S3 cuff signals respectively. In 2 animals small spontaneous bladder contractions were clearly reflected in the pelvic nerve signal (contractions were observed only in 3 pigs). Except in one pig, there were no slow/fast bladder filling responses recorded in the S2 roots. It is shown that the recorded responses were afferent.Conclusions: Cuff electrodes can be used to record bladder afferent information from the pelvic nerve and the sacral root S3 in pig. Pelvic nerve recordings were more selective than the sacral root recordings. Nerve activity increases were more distinct and repeatable during rapid bladder pressure changes and small spontaneous bladder contractions than during slow bladder fillings.