Independent patterns of cytochrome P450 gene expression in liver and blood in patients with suspected liver disease-猫眼课题宝

疑似肝病患者肝脏和血液中细胞色素 P450 基因表达的独立模式

DOI：

发表时间：

2001

期刊：

European Journal of Clinical Pharmacology

影响因子：

2.9

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

N. Finnström;M. Thörn;L. Lööf;A. Rane

抽象的。目的：通过比较来自13个个体的配对肝脏和血液样本中的细胞色素P450（CYP 1A 1）基因表达，使用血液样本评估肝脏代谢。方法：从经皮穿刺活检组织中提取总RNA，同时采集血液。使用实时逆转录聚合酶链反应（RT-PCR）方法研究了CYP 1A 2、CYP 1B 1、CYP 2 E 1和CYP 3A 4的基因表达。结果：所有的肝活检组织中均有不同程度的表达。分别观察到CYP 2 E1和CYP 1B 1的最高和最低表达水平。在血液和肝脏中的表达模式不同。CYP 1B 1在所有血液样本中的表达水平比肝脏高20%。CYP 1A 2、CYP 2 E1和CYP 3A 4在血液中的表达水平比在肝脏中低35- 5000倍。血液中的转录本与肝脏中的表达没有任何相关性。结论：我们的结论是，血液不能作为替代器官的研究在肝脏中的表达的研究的p53基因的评估。

Abstract. Objective: Assessment of liver metabolism using blood samples was tested by comparison of cytochrome P450 (CYP) gene expression in paired liver and blood samples from 13 individuals. Methods: Total RNA was isolated from percutaneous needle biopsies and blood collected simultaneously. Gene expression for CYP1A2, CYP1B1, CYP2E1 and CYP3A4 was studied using a real-time reverse-transcription polymerase chain reaction (RT-PCR) method. Results: All CYP mRNA species were expressed in all liver biopsies but at varying levels. The highest and lowest levels of expression were observed for CYP2E1 and CYP1B1, respectively. The expression patterns differed between blood and liver. CYP1B1 was expressed in all blood samples at a 20% higher level than in the liver. CYP1A2, CYP2E1 and CYP3A4 were expressed in blood at 35- to 5000-fold lower levels than in liver. None of the transcripts in blood showed any correlation with the expression in liver. Conclusion: We conclude that blood cannot serve as a surrogate organ for assessment of the expression of the studied CYP genes in liver.