Comparison of Batch Mode and Dynamic Physiologically Based Bioaccessibility Tests for PAHs in Soil Samples
Comparison of Batch Mode and Dynamic Physiologically Based Bioaccessibility Tests for PAHs in Soil Samples
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DOI：
10.1021/es903258v
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发表时间：
2010-04-01
影响因子：
11.4
通讯作者：
Daly, Paddy
中科院分区：
文献类型：
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作者：
Cave, Mark R.;Wragg, Joanna;Daly, Paddy
开发了一种可用于商业测试实验室的FED状态体外方法，用于测量土壤中多环芳烃(PAHs)的人体摄取生物可及性(FED有机估计人体模拟测试-FOREhST)。测定模拟胃肠液中多环芳烃的方法使用甲醇氢氧化钾皂化，然后结合聚合物吸附剂固相萃取和二氧化硅吸附小柱进行样品清理和预浓缩。采用高效液相色谱-荧光检测法进行分析。通过测定6种多环芳烃(苯并[a]菲、苯并[b]荧菲、苯并[k]荧菲、苯并[a]芘、二苯并[ah]菲和吲哚并[1，2，3-c，d]芘)在11个气田土壤中的生物可及性，评价了该方法的重复性，RSD为10%。该方法与在相同土壤上测试的由胃、十二指肠和结肠隔间组成的独立动态人体模拟反应堆的结果进行了很好的比较。在土壤中总有机碳含量为1-13%、多环芳烃含量为10-300 mg kg(-1)的土壤中，测得的生物可及分数为10-60%。多元回归模型表明，多环芳烃生物可及性分数可以用多环芳烃化合物、土壤类型和多环芳烃总量与土壤有机碳含量来解释。本文描述的方法有可能对特定地点进行详细的定量风险评估，或者修改风险估计或对风险评估做出贡献。
A fed state in vitro methodology capable of use in commercial testing laboratories has been developed for measuring the human ingestion bioaccessibility of polyaromatic hydrocarbons (PAHs) in soil (Fed ORganic Estimation human Simulation Test-FOREhST). The protocol for measuring PAHs in the simulated gastro-intestinal fluids used methanolic KOH saponification followed by a combination of polymeric sorbent solid phase extraction and silica sorbent cartridges for sample cleanup and preconcentration. The analysis was carried out using high pressure liquid chromatography with fluorescence detection. The repeatability of the method, assessed by the measurement of the bioaccessibility of 6 PAHs (benz[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenz[ah]anthracene, and indeno[1,2,3-c, d]pyrene) in eleven gas works soils, was 10% RSD. The method compared well with the results from an independent dynamic human simulation reactor comprising of the stomach, duodenal and colon compartments tested on the same soils. The measured bioaccessible fraction of the soils varied from 10-60% for soils containing 10-300 mg kg(-1) PAH (the sum of the six studied) with total organic carbon concentrations in the soils ranging from 1-13%. A multiple regression model showed that the PAH bioaccessible fraction could be explained using the PAH compound, the soil type and the total PAH to soil organic carbon content The method described here has potential for site specific detailed quantitative risk assessment either to modify the risk estimation or to contribute to the risk evaluation.