Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction
Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction
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DOI：
10.1103/physrevlett.102.018101
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发表时间：
2009-01-09
影响因子：
8.6
通讯作者：
Maeshima, Kazuhiro
中科院分区：
文献类型：
--
作者：
Nishino, Yoshinori;Takahashi, Yukio;Maeshima, Kazuhiro
相干X射线衍射显微术是一种具有高图像对比度的无透镜相位衬度成像技术。尽管电子断层扫描能够深入研究细胞器的三维结构，但对于厚的物体存在固有困难。X射线具有独特的优势，能够对较厚的物体进行无创分析，并具有高空间分辨率。我们使用相干X射线衍射观察了未染色的人类染色体。二维或三维重建图像显示出一种轴向结构，这在未染色的条件下是未曾观察到的。
Coherent x-ray diffraction microscopy is a lensless phase-contrast imaging technique with high image contrast. Although electron tomography allows intensive study of the three-dimensional structure of cellular organelles, it has inherent difficulty with thick objects. X rays have the unique benefit of allowing noninvasive analysis of thicker objects and high spatial resolution. We observed an unstained human chromosome using coherent x-ray diffraction. The reconstructed images in two or three dimensions show an axial structure, which has not been observed under unstained conditions.