The C-type lectin DC-SIGN (CD209) is an antigen-uptake receptor for Candida albicans on dendritic cells
The C-type lectin DC-SIGN (CD209) is an antigen-uptake receptor for Candida albicans on dendritic cells
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DOI：
10.1002/immu.200310029
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发表时间：
2003-02-01
影响因子：
5.4
通讯作者：
Figdor, CG
中科院分区：
文献类型：
--
作者：
Cambi, A;Gijzen, K;Figdor, CG
表达II型C型凝集素DC-SIGN(CD209)的树突状细胞(DC)位于组织的粘膜下层，在那里它们介导HIV-1的进入。有趣的是，白色念珠菌是医院获得性真菌感染的主要原因，它在类似的粘膜下部位穿透。在这里，我们证明了DC-SIGN能够在DC-SIGN转基因细胞系和人单核细胞来源的DC中结合白色念珠菌。结合被证明是时间和浓度依赖的，活的和热灭活的白色念珠菌结合的程度是相同的。此外，在未成熟的DC中，DC-SIGN能够将白念珠菌内化在特定的DC-SIGN富集泡中，这与那些含有甘露糖受体的囊泡不同，甘露糖受体是DC表达的另一种已知的白色念珠菌受体。综上所述，这些结果表明DC-SIGN是一种精致的病原体摄取受体，不仅能捕获病毒，还能捕获真菌。
Dendritic cells (DC) that express the type II C-type lectin DC-SIGN (CD209) are located in the submucosa of tissues, where they mediate HIV-1 entry. Interestingly, the pathogen Candida albicans, the major cause of hospital-acquired fungal infections, penetrates at similar submucosal sites. Here we demonstrate that DC-SIGN is able to bind C. albicans both in DC-SIGN-transfected cell lines and in human monocyte-derived DC. The binding was shown to be time- as well as concentration-dependent, and live as well as heat-inactivated C. albicans were bound to the same extent. Moreover, in immature DC, DC-SIGN was able to internalize C. albicans in specific DC-SIGN-enriched vesicles, distinct from those containing the mannose receptor, the other known C. albicans receptor expressed by DC. Together, these results demonstrate that DC-SIGN is an exquisite pathogen-uptake receptor that captures not only viruses but also fungi.