Contrasting effects of different maternal diets on sexually dimorphic gene expression in the murine placenta
Contrasting effects of different maternal diets on sexually dimorphic gene expression in the murine placenta
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DOI：
10.1073/pnas.1000440107
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发表时间：
2010-03-23
影响因子：
11.1
通讯作者：
Rosenfeld, Cheryl S.
中科院分区：
文献类型：
--
作者：
Mao, Jiude;Zhang, Xia;Rosenfeld, Cheryl S.
怀孕期间的饮食会影响女性后代未来的健康，结果因孩子的性别而异。由于胎盘缓冲胎儿从母亲，我们研究了饮食和胎儿性别对胎盘基因表达的影响，无论是非常高脂肪，低脂肪，食物饮食的中间热量密度的小鼠。在妊娠第12.5天，提取胎盘RNA并通过微阵列分析。在三个组中的至少一个组中，1，972个基因的表达在不同饮食的比较中变化超过2倍（P < 0.05）。女性胎盘表现出更显着的基因表达的变化，在母亲的饮食比男性胎盘。值得注意的是，每种饮食提供了一个独特的签名的性别二型基因，与表达一般较高的基因（651 700）从女性胎盘比男性胎盘。几个基因通常被认为是肾功能的特征受到饮食的影响，包括调节离子平衡和化学感受的基因。胎盘还表达了大多数已知的嗅觉受体基因（Olfr），这可能使胎盘能够感知气味分子和其他次要的饮食成分，其中许多基因的转录水平受到饮食和胎儿性别的影响。总之，小鼠胎盘中的基因表达是适应性的，并通过母体饮食形成。它还表现出明显的性别二型性，与胎盘的女性更敏感的营养扰动比胎盘的男性。
Diet during pregnancy influences the future health of a woman's offspring, with outcomes differing depending on the child's sex. Because the placenta buffers the fetus from the mother, we examined the impact of diet and fetal sex on placental gene expression in mice fed either a very-high-fat, low-fat, chow diet of intermediate caloric density. At day 12.5 of pregnancy, placental RNA was extracted and analyzed by microarray. The expression of 1,972 genes was changed more than 2-fold (P < 0.05) in comparisons across diet in at least one of the three groups. Female placentae demonstrated more striking alterations in gene expression in response to maternal diet than male placentae. Notably, each diet provided a distinctive signature of sexually dimorphic genes, with expression generally higher in genes (651 out of 700) from female placentae than those from male placentae. Several genes normally considered as characteristic of kidney function were affected by diet, including genes regulating ion balance and chemoreception. The placenta also expressed most of the known olfactory receptor genes (Olfr), which may allow the placenta to sense odorant molecules and other minor dietary components, with transcript levels of many of these genes influenced by diet and fetal sex. In conclusion, gene expression in the murine placenta is adaptive and shaped by maternal diet. It also exhibits pronounced sexual dimorphism, with placentae of females more sensitive to nutritional perturbations than placentae of males.