INCORPORATION OF THYMIDINE AND LEUCINE IN THE SUB-ARCTIC PACIFIC - APPLICATION TO ESTIMATING BACTERIAL PRODUCTION
INCORPORATION OF THYMIDINE AND LEUCINE IN THE SUB-ARCTIC PACIFIC - APPLICATION TO ESTIMATING BACTERIAL PRODUCTION
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DOI：
10.3354/meps082301
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发表时间：
1992-06-01
影响因子：
2.5
通讯作者：
KIRCHMAN, DL
中科院分区：
文献类型：
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作者：
KIRCHMAN, DL
本研究考察了胸腺嘧啶（TdR）和亮氨酸（Leu）掺入率的使用，以估计亚北极太平洋海洋中的细菌产量。不同浓度下的掺入率表明，当使用5 nM TdR时，同位素稀释度较低，但其他数据表明，TdR和Leu的稀释度分别至少为3倍和2倍。与冷酸不溶性组分相比，热酸不溶性组分中TdR的掺入率为0.66，Leu的掺入率为0.86。在4次1公里长的巡航中进行了10次实验，测量了经验转换因子。转化因子没有系统变化，也不受添加有机化合物或铵的影响。合并TdR的平均转化因子为1.74 × 10（18）个细胞mol-1 (SD = 0.89 × 10（18）个细胞mol-1)，合并Leu的平均转化因子为0.108 × 10（18）个细胞mol-1 （SD = 0.085 × 10（18）个细胞mol-1）。1988年5月，当细菌数量大幅增加时，可以估计“无瓶”转换系数；TdR和Leu的原位无瓶转化因子分别为1.55和0.185 × 10(18) cells mol-1。基于TdR和Leu方法的细菌产量估计分别在0至40 m（混合层）和40至80 m（光区下半部分）的10%和25%范围内一致。
This study examines the use of incorporation rates of thymidine (TdR) and leucine (Leu) in order to estimate bacterial production in the oceanic subarctic Pacific. Incorporation rates at different concentrations suggest that isotope dilution is low when 5 nM TdR is used, but other data indicate at least 3-fold and 2-fold dilution for TdR and Leu, respectively. The ratio of incorporation into the hot acid insoluble fraction, compared to the cold acid insoluble fraction, is 0.66 for TdR and 0.86 for Leu. Empirical conversion factors were measured in 10 experiments during four 1 mo-long cruises. Conversion factors did not vary systematically and were not affected by additions of organic compounds or ammonium. Average conversion factors were 1.74 X 10(18) cells mol-1 of incorporated TdR (SD = 0.89 X 10(18) cells mol-1) and 0.108 X 10(18) cells mol-1 of incorporated Leu (SD = 0.085 X 10(18) cells mol-1). It was possible to estimate 'bottle-less' conversion factors during May 1988 when bacterial numbers increased greatly; in situ bottle-less conversion factors were 1.55 and 0.185 X 10(18) cells mol-1 for TdR and Leu, respectively. Estimates of bacterial production based on the TdR and Leu methods agreed within 10 and 25 % for 0 to 40 m (ca the mixed layer) and 40 to 80 m (bottom half of the euphotic zone), respectively.