Fructus Ligustri Lucidi aqueous extract promotes calcium balance and short-chain fatty acids production in ovariectomized rats
Fructus Ligustri Lucidi aqueous extract promotes calcium balance and short-chain fatty acids production in ovariectomized rats
复制标题
女贞子水提取物促进去势大鼠的钙平衡和短链脂肪酸的产生
DOI：
10.1016/j.jep.2021.114348
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发表时间：
2021-06-21
影响因子：
5.4
通讯作者：
Zhang, Dongwei
中科院分区：
文献类型：
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作者：
Chen, Beibei;Wei, Junping;Zhang, Dongwei
民族药理学相关性：女贞子（Fructus Ligustri Lucidi，FLL）是一种具有抗骨质疏松活性的可食用草药，但其水提物是否以及如何影响钙代谢以保持骨质量尚不清楚。研究目的：目的：探讨FLL水提物对去卵巢大鼠钙平衡及短链脂肪酸（SCFAs）生成的影响。材料与方法：去卵巢大鼠每日灌胃FLL水提物3.5g/kg，连续14周。ELISA法检测大鼠血清中Ⅰ型胶原N端前肽（PINP）和Ⅰ型胶原C端肽（CTx-Ⅰ）的含量。血清、尿液和粪便中的钙浓度通过生化测定来确定。通过Micro-CT、三点弯曲试验和傅里叶变换红外（FTIR）光谱测定骨质量。采用免疫组化、Western blotting和实时荧光定量PCR方法检测肾脏钙结合蛋白D28 K和钙敏感受体（CaSR）以及十二指肠维生素D受体（VDR）、瞬时受体电位香草酸受体6（TRPV6）和钙结合蛋白D9k的表达。采用气相色谱法测定盲肠粪便中短链脂肪酸（SCFAs）的含量。结果如下：给OVX大鼠施用FLL导致骨矿物质密度和生物力学强度的显着改善，以及保持骨微观结构和材料质量。同时，FLL可恢复OVX大鼠PINP水平的下降和CTx-I水平的升高。此外，FLL治疗增加了钙的吸收，上调了VDR，TRPV 6，钙结合蛋白D9k在十二指肠的表达，钙结合蛋白D28K在肾脏中的表达，下调了CaSR在肾脏中的表达，以及增加了OVX大鼠粪便中的SCFAs水平。结论：FLL水提物可能通过调节去卵巢大鼠的钙平衡和肠道SCFAs的产生来保护骨质量。这为FLL在骨质疏松症临床试验中的应用提供了参考价值。
Ethnopharmacological relevance: Fructus Ligustri Lucidi (FLL) is an edible herb with anti-osteoporotic activity, yet whether and how the aqueous extract of this herb affect calcium metabolism in preservation of bone quality remain unclear. Aim of the study: To investigate the effects of FLL aqueous extract on calcium balance and short-chain fatty acids (SCFAs) production in ovariectomized (OVX) rats. Materials and methods: OVX rats were daily and orally administrated with FLL aqueous extract (3.5 g/kg) for 14 weeks. The levels of N-terminal propeptide of type I collagen (PINP) and C-terminal telopeptide of type I collagen (CTx-I) in rat serum were evaluated by ELISA assays. The concentration of calcium in serum, urine, and feces were determined by biochemical assays. Bone quality was determined by Micro-CT, a three-point bending assay, and Fourier Transform Infrared (FTIR) Spectrometry. The expressions of Calbindin D28K and Calcium-sensing receptor (CaSR) in kidney as well as the Vitamin D receptor (VDR), the transient receptor potential vanilloid receptor 6 (TRPV6), Calbindin D9k in the duodenum were measured by immunohistochemistry, western blotting, or real-time PCR. The short-chain fatty acids (SCFAs) levels in the feces of the cecum were tested by gas chromatograghy. Results: The administration of FLL to OVX rats resulted in a significant improvement in bone mineral density and biomechanical strength as well as in maintaining bone microstructures and material quality. Meanwhile, the decreased levels of PINP and increased levels of CTx-I in OVX rats were restored by FLL treatment. Additionally, FLL treatment increased calcium absorption, upregulated VDR, TRPV6, Calbindin D9k expressions in the duodenum, Calbindin D28K in kidney, and down-regulated CaSR expression in the kidney, as well as enhanced SCFAs levels in the feces of OVX rats. Conclusions: FLL aqueous extract may preserve bone quality through regulation of the calcium balance and intestinal SCFAs production in OVX rats. This offers translational value of FLL into osteoporosis clinical trial.