RESPIRATION IN VITRO OF SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEX
RESPIRATION IN VITRO OF SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEX
复制标题
DOI：
10.1111/j.1471-4159.1969.tb06444.x
复制
发表时间：
1969-01-01
影响因子：
4.7
通讯作者：
BRADFORD, HF
中科院分区：
文献类型：
--
作者：
BRADFORD, HF
—（1）比较哺乳动物大脑皮层突触体和线粒体的呼吸特性。（2）在克雷布斯-林格培养基中，以葡萄糖和丙酮酸为底物，突触体表现出高且线性的呼吸。线粒体只有在缺Na、高钾和磷酸盐的培养基中以丙酮酸为底物时才表现出这种呼吸作用。(3)突触体暴露于低渗介质中导致乳酸脱氢酶(LDH)和蛋白质的损失，呼吸减弱并变得非线性。(4)ATP和磷酸肌酸都是由突触体以葡萄糖为底物合成的。 ATP在丙酮酸存在下由线粒体合成。(5)突触体显示出一定的主动积累钾的能力，而线粒体没有。(6)线粒体和突触体都以谷氨酸为底物进行呼吸。谷氨酸会导致突触体中 80% 的 ATP 和磷酸肌酸损失，但不会降低线粒体 ATP 水平。
—(1) The respiratory properties of synaptosomes and mitochondria from mammalian cerebral cortex are compared.(2) Synaptosome showed high and linear respiration with glucose and pyruvate as substrates in Krebs‐Ringer media. Mitochondria showed such respiration only with pyruvate as substrate in media lacking Na and high in K and phosphate.(3) Exposure of synaptosomes to hypotonic media caused loss of lactate dehydrogenase (LDH) and protein, and respiration diminished and became non‐linear.(4) Both ATP and phosphocreatine were synthesised by synaptosomes with glucose as substrate. ATP was synthesised by mitochondria in the presence of pyruvate.(5) Synaptosome but not mitochondria showed some capacity for active accumulation of potassium.(6) Both mitochondria and synaptosomes respired with glutamate as substrate. Glutamate caused 80 per cent loss of ATP and phosphocreatine in synaptosomes but did not diminish the level of mitochondrial ATP.