Multifunctional nanostructures based on inorganic nanoparticles and oligothiophenes and their exploitation for cellular studies
Multifunctional nanostructures based on inorganic nanoparticles and oligothiophenes and their exploitation for cellular studies
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DOI：
10.1021/ja800102v
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发表时间：
2008-08-13
影响因子：
15
通讯作者：
Pellegrino, Teresa
中科院分区：
文献类型：
--
作者：
Quarta, Alessandra;Di Corato, Riccardo;Pellegrino, Teresa
将具有不同性质（例如荧光和磁性）的材料组合成一个纳米级尺寸的单一物体对生物技术来说是一个有吸引力的挑战，特别是因为它们在生物医学应用中的潜在相关性。在这里，我们报告的基础上的无机纳米粒子的有机寡聚噻吩荧光团（OTFs）的连接，制备新的双功能共轭物。与生物成像中常用的有机染料相比，OTF更类似于胶体量子点，具有宽的光学吸收光谱，因此可以用单个激发源激发以不同颜色发射的OTF荧光团，从而允许更容易的多路复用分析。在这项工作中，我们展示了基于金和氧化铁纳米颗粒的OTF-纳米颗粒缀合物的制备及其表征，使用不同的技术，如凝胶电泳，光致发光光谱，动态光散射，等等。此外，通过对人类肿瘤细胞进行体外研究，我们表明，OTF-纳米颗粒缀合物可以发射不同的颜色用于多重检测。此外，在氧化铁-OTF缀合物的情况下，一旦被细胞摄取，我们表明它们保留了它们的荧光和磁性。
The combination of materials that possess different properties (such as, for instance, fluorescence and magnetism) into one single object of nanoscale size represents an attractive challenge for biotechnology, especially for their potential relevance in biomedical applications. We report here the preparation of novel bifunctional conjugates based on the linkage of inorganic nanoparticles to organic oligothiophene fluorophores (OTFs). In comparison to the organic dyes commonly used in bioimaging and more similarly to colloidal quantum dots, OTFs have broad optical absorption spectra, and therefore OTF fluorophores emitting at different colors can be excited with a single excitation source, allowing for easier multiplexing analysis. In this work we show the preparation of OTF-nanoparticle conjugates based on gold and iron oxide nanoparticles and their characterization using different techniques such as gel electrophoresis, photoluminescence spectroscopy, dynamic light scattering, and so on. In addition, by performing an in vitro study on human tumor cells we show that OTF-nanoparticle conjugates emitting at different colors can be used for multiplexing detection. Also, in the case of iron oxide-OTF conjugates, once uptaken by the cells, we show that they preserve both their fluorescent and their magnetic properties.