Mitochondrial DNA sequence variation of four Saccharina species (Laminariales, Phaeophyceae) growing in Japan
Mitochondrial DNA sequence variation of four Saccharina species (Laminariales, Phaeophyceae) growing in Japan
复制标题
DOI：
10.1007/s10811-009-9452-7
复制
发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Druehl, Louis D.
中科院分区：
文献类型：
--
作者：
Yotsukura, Norishige;Shimizu, Takeshi;Druehl, Louis D.
对四种糖类的完整线粒体 DNA (mtDNA) 进行了测序，其中包括北海道的三种糖类：S. japonica；S. japonica；S. japonica；S. japonica；S. japonica；S. japonica；粳稻变种宗教；粳稻变种兔紧张症；粳稻变种迪亚波利卡；长足糖精；狭糖糖；和糖精。此外，还对它们之间的结构和序列进行了比较。总核苷酸长度为37,500-37,657 bp。所有 mtDNA 都被绘制出来，并且没有发现编码区组织的差异。从包括3个品种在内的粳稻和长足粳稻的全基因组比对中，在rps19上检测到68个位点的核苷酸取代，并在1个位点检测到核苷酸插入/缺失。用于区分 S. japonica 品种的可变区是 trnI、trnM、rps19、ORF41 和七个间隔区。 S. japonica、S. angustata 和 S. coraiacea 之间的配对距离为 0.000-0.116。 rps7、rps11、rpl5 和 tatC 的测序比较被认为是对具有相似形态的糖类物种进行区分和系统发育分析的有用工具。
The complete mitochondrial DNA (mtDNA) was sequenced for four Saccharina species including three varieties of Saccharina japonica in Hokkaido: S. japonica; S. japonica var. religiosa; S. japonica var. ochotensis; S. japonica var. diabolica; Saccharina longipedalis; Saccharina angustata; and Saccharina coriacea. Furthermore, the structure and the sequence were compared among them. The total nucleotide length was 37,500-37,657 bp. All mtDNAs were mapped and no differences in the organization of the coding region were found. From the total alignment of S. japonica including the three varieties and S. longipedalis, nucleotide substitutions were detected at 68 sites, and a nucleotide insertion/deletion was detected at one site on rps19 for the whole genome. Variable regions useful for varieties distinction of S. japonica were trnI, trnM, rps19, ORF41, and seven spacers. The pairwise distance between S. japonica, S. angustata, and S. coraiacea was 0.000-0.116. Sequencing comparisons of rps7, rps11, rpl5, and tatC were thought to be useful tools for discrimination and phylogenetic analysis of Saccharina species having similar morphologies.