Ontogeny of myocardial alpha 1-adrenergic receptor in the rat.
Ontogeny of myocardial alpha 1-adrenergic receptor in the rat.
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大鼠心肌α1-肾上腺素能受体的个体发育。
DOI：
10.1159/000457439
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Dickman,L
中科院分区：
文献类型：
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作者：
Noguchi,A;Whitsett,JA;Dickman,L
α-肾上腺素能特异性放射性配体[3H]-哌唑嗪和β-肾上腺素能放射性配体(-)-[3H]-二氢阿普洛尔[(-)-[3H]-DHA]用于描述大鼠心室心肌颗粒部分中α(1)-和β-肾上腺素能受体的出生后个体发育。在出生后的前 15 天内，[3H]-哌唑嗪结合位点的数量从 35 ± 9 增加到 163 ± 5 fmol • mg^-1 蛋白质 (p < 0.01)，增加了 4 至 5 倍，此后随着年龄的增长逐渐减少，在成年心肌中达到 83 ± 5 fmol • mg^-1 蛋白质。(-)-[3H]-DHA 与同一组织的结合在出生 1 天后，新生儿的蛋白质含量从 61 ± 6 增至成人的 37 ± 4 fmol • mg-1 (p < 0.01)。 [3H]-哌唑嗪或(-)-[3H]-DHA 的亲和力 (K(D)) 分别约为 0.13 和 1.8 nM，并且不随年龄的增长而变化。我们得出的结论是，大鼠心室心肌中α(1)-肾上腺素能受体的出生后发育不同于β-肾上腺素能受体。讨论了与心脏交感神经支配和心脏生长有关的这种差异的可能解释。
The ai-adrenergic specific radioligand [3H]-prazosin and the ß-adrenergic radioligand (-)-[3H]-dihydroalprenolol [(-)-[3H]-DHA] were used to describe the postnatal ontogeny of α(1)- and β-adrenergic receptors in particulate fractions of rat ventricular myocardium. The number of [3H]-prazosin binding sites increased 4- to 5-fold during the first 15 days of age from 35 ± 9 to 163 ± 5 fmol • mg^-1 protein (p < 0.01), and thereafter decreased progressively with age, reaching 83 ± 5 fmol • mg^-1 protein in the adult myocardium.(-)-[3H]-DHA binding to the same tissue decreased progressively at 1 day of age from 61 ± 6 in the newborn to 37 ± 4 fmol • mg-1 protein in the adult (p < 0.01). Affinities (K(D)) for [3H]-prazosin or (-)-[3H]-DHA were approximately 0.13 and 1.8 nM,respectively, and did not change with advancing age. We conclude that the postnatal development of the α(1)-adrenergic receptors is distinct from that of the β-adrenergic receptor in the rat ventricular myocardium. Possible explanations for this difference are discussed in relation to cardiac sympathetic innervation and cardiac growth.