The high-affinity phosphate-binding protein PstS is accumulated under high fructose concentrations and mutation of the corresponding gene affects differentiation in Streptomyces lividans
The high-affinity phosphate-binding protein PstS is accumulated under high fructose concentrations and mutation of the corresponding gene affects differentiation in Streptomyces lividans
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DOI：
10.1099/mic.0.27983-0
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发表时间：
2005-08-01
期刊：
影响因子：
2.8
通讯作者：
Santamaría, RI
中科院分区：
文献类型：
--
作者：
Díaz, M;Esteban, A;Santamaría, RI
变铅青链霉菌的分泌蛋白模式取决于培养基中存在的碳源。显示出最显著变化的一种蛋白质是高亲和力磷酸盐结合蛋白PstS，其在含有高浓度（> 3%）的某些糖（如果糖、半乳糖和甘露糖）的液体培养物的上清液中强烈积累。当在果糖存在下生长时，在培养物上清液中积累的变青聚糖。PstS积累在S.在缺乏phoP的缺失突变体中受损，phoP是控制Pho调节子的双组分phoR-phoP系统的转录调节基因。在S. lividans和S.在固体培养基上，腔棘鱼损害磷酸盐运输并加速分化和产孢。与S中的单个拷贝互补。变铅青蛋白pstS无效突变体使磷酸盐转运和孢子形成恢复到与野生型菌株相似的水平。目前的工作表明，碳和磷酸盐代谢在基因调控中是相互联系的，这可以触发形态发生的遗传开关。
The secreted protein pattern of Streptomyces lividans depends on the carbon source present in the culture media. One protein that shows the most dramatic change is the high-affinity phosphate-binding protein PstS, which is strongly accumulated in the supernatant of liquid cultures containing high concentrations (> 3 %) of certain sugars, such as fructose, galactose and mannose, The promoter region of this gene and that of its Streptomyces coelicolor homologue were used to drive the expression of a xylanase in S. lividans that was accumulated in the culture supernatant when grown in the presence of fructose. PstS accumulation was dramatically increased in a S. lividans polyphosphate kinase null mutant (Delta ppk) and was impaired in a deletion mutant lacking phoP, the transcriptional regulator gene of the two-component phoR-phoP system that controls the Pho regulon. Deletion of the pstS genes in S. lividans and S. coelicolor impaired phosphate transport and accelerated differentiation and sporulation on solid media. Complementation with a single copy in a S. lividans pstS null mutant returned phosphate transport and sporulation to levels similar to those of the wild-type strain. The present work demonstrates that carbon and phosphate metabolism are linked in the regulation of genes and that this can trigger the genetic switch towards morphogenesis.