High levels of ferritin and its iron in fetal bovine serum
High levels of ferritin and its iron in fetal bovine serum
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DOI：
10.1016/s0300-9629(96)00403-3
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发表时间：
1997-09-01
影响因子：
2.3
通讯作者：
Watanabe, K
中科院分区：
文献类型：
--
作者：
Kakuta, K;Orino, K;Watanabe, K
在13批商业胎牛血清中，铁蛋白水平在0.8和6.0 μ g/ml之间。通过定量免疫沉淀技术测定的血清铁蛋白铁浓度范围为0.16 - 0.96 μ g/ml，铁蛋白的铁含量约为20%，与血清中的蛋白质浓度无关。血清铁蛋白占血清总铁的百分比为8.8 ~ 28.5%，与血清铁蛋白浓度呈显著正相关（r = 0.9368，P < 0.001）。胎儿血清中的铁蛋白与伴刀豆球蛋白A结合的比例不显著。免疫印迹结果表明，胎血清铁蛋白L（艾弗）和H（心脏）型亚基的分子量分别与成年牛脾铁蛋白L和H亚基的分子量相同（L：21 kDa，H：18 kDa），且L亚基在血清蛋白中占优势。血清转铁蛋白水平相对恒定（1.8-2.2 mg/ml），而转铁蛋白饱和度从54.8%到91.7%不等。血清铁蛋白浓度与转铁蛋白饱和度呈显著相关（r = 0.8864，P < 0.001）。这些结果表明，牛胎儿具有较高的铁储备。(C)1997年爱思唯尔科学公司
In 13 lots of the commercial fetal bovine sera, the ferritin levels ranged between 0.8 and 6.0 mu g/ml. The serum ferritin iron concentration as measured by a quantitative immunoprecipitation technique ranged from 0.16 to 0.96 mu g/ml, and the iron content of ferritin was about 20% regardless of its protein concentration in sera. The percentage of ferritin iron to total serum iron ranged from 8.8 to 28.5%, and correlated significantly with ferritin concentration (r = 0.9368, P < 0.001). No significant proportion of the ferritin in fetal serum bound to concanavalin A. Immunoblotting showed that the molecular weights of L(iver)- and H(eart)type subunits of fetal serum ferritin were identical to those of L and H subunits of adult bovine spleen ferritin (L:21kDa, H:18kDa), respectively, and that the L subunit predominated in the serum protein. Serum transferrin level was relatively constant (1.8-2.2 mg/ml), whereas transferrin saturation varied from 54.8 to 91.7%. There was a significant correlation between serum ferritin concentration and transferrin saturation (r = 0.8864, P < 0.001). These findings demonstrate that the bovine fetuses have the elevated iron stores. (C) 1997 Elsevier Science Inc.