The Hordeum vulgare signalling protein MAP kinase 4 is a regulator of biotic and abiotic stress responses
The Hordeum vulgare signalling protein MAP kinase 4 is a regulator of biotic and abiotic stress responses
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DOI：
10.1016/j.jplph.2013.04.009
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发表时间：
2013-10-15
影响因子：
4.3
通讯作者：
Morris, Peter C.
中科院分区：
文献类型：
--
作者：
Abass, Mohammed;Morris, Peter C.
丝裂原活化蛋白激酶（MAP激酶）信号转导途径是调控细胞应激反应的重要真核机制。这项工作的目的是研究大麦MAP激酶HvMPK4（拟南芥MAP激酶AtMPK1的同源物）在植物对生物和非生物胁迫的反应中的作用。结果表明，HvMPK4基因在反义系和过表达系中的表达量显著降低，在过表达系中的表达量约为2倍。每个构建的三个独立的系测试了它们对真菌病原体和盐处理的反应。反义系对半生物营养真菌病原菌Magnaporthe grisea具有较强的抗性，感染后水杨酸（SA）和过氧化氢水平升高；因此，HvMPK4是感染后SA产生的负调节因子。感染后，过表达系茉莉酸和乙烯水平均升高，但对病原菌的抗性并未增强。然而，过表达系对非生物胁迫表现出更强的耐受性，在盐处理2周后，这些系的鲜重和干重减少较少，叶片中积累的盐分较少，渗透保护氨基酸脯氨酸含量增加。(C) 2013爱思唯尔有限公司版权所有。
Mitogen activated protein kinase (MAP kinase) signal transduction pathways are important eukaryotic mechanisms for regulating cellular responses to stress. The objective of this work was to investigate the role of the barley MAP kinase HvMPK4 (a homologue of the Arabidopsis MAP kinase AtMPK1) in the plant response to biotic and abiotic stress. Transgenic barley plants bearing antisense or overexpression constructs for HvMPK4 were produced, and RNA blot analysis showed that HvMPK4 gene expression was much reduced in the antisense lines and approximately double in the overexpression lines. Three independent lines of each construct were tested for their response to a fungal pathogen and to salt treatment. The antisense lines were more resistant to the hemibiotrophic fungal pathogen Magnaporthe grisea, and showed enhanced levels of salicylic acid (SA) and of hydrogen peroxide following infection; HvMPK4 is thus a negative regulator of SA production post infection. The overexpression lines had constitutively higher levels of jasmonic acid and enhanced levels of ethylene following infection but were not more resistant to the pathogen. However the overexpression lines showed greater tolerance to abiotic stress, as following 2 weeks of salt treatment these lines showed less reduction in fresh and dry weight, accumulated less salt in the leaves and contained enhanced levels of the osmoprotectant amino acid, proline. (C) 2013 Elsevier GmbH. All rights reserved.