Enhancement of Natural Killer Cell Activity in Healthy Subjects by Immulina®, a Spirulina Extract Enriched for Braun-Type Lipoproteins
Enhancement of Natural Killer Cell Activity in Healthy Subjects by Immulina®, a Spirulina Extract Enriched for Braun-Type Lipoproteins
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DOI：
10.1055/s-0030-1250043
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发表时间：
2010-11-01
期刊：
影响因子：
2.7
通讯作者：
Pasco, David S.
中科院分区：
文献类型：
--
作者：
Nielsen, Claus Henrik;Balachandran, Premalatha;Pasco, David S.
Im ulina(R)是钝顶螺旋藻(Spirulina)的商业提取物，在体外对THP-1单核细胞和CD4+T细胞具有强大的激活剂作用，并能增强小鼠的多种免疫功能。我们通过确定布劳恩型脂蛋白是这种材料表现出的体外单核细胞激活的主要原因，进一步表征了IMMULINA(R)。为了了解Immulina(R)对NK细胞活性的影响，对10名健康的北美人进行了一项初步研究，这些人在饮食中添加了Immulina(R)(每天400毫克)7天。我们观察到在补充免疫(R)后，NK细胞对K562肿瘤细胞的杀伤率平均增加了40%(p<；0.01)。在另一项涉及11名丹麦健康受试者的安慰剂对照的交叉研究中，我们观察到，在服用Immulina(R)(分别为每天200毫克和400毫克)7天后，NK细胞标记物NKG2DmRNA的表达增加了37%(p=0.02)和55%(p=0.0003)。每天给予免疫球蛋白400 mg后，NK细胞和T细胞标记物穿孔素的表达增加了75%(p=0.008)。两种标志物均表现出明显的剂量依赖效应(p=0.0003.0 1和p=0.0 2)。免疫球蛋白对CD56(亮)/CD56(暗)NK细胞的比例无影响。总而言之，两项独立的研究表明，服用Immulina(R)7天后，NK细胞活性增强。
Immulina (R), a commercial extract of Arthrospira (Spirulina) platensis is a potent activator of THP-1 monocytes and CD4+ T cells in vitro and enhances several immunological functions in mice. We further characterized Immulina (R) by determining that Braun-type lipoproteins are responsible for a major portion of the in vitro monocyte activation exhibited by this material. In order to understand the effect of Immulina (R) on NK cell activity, a pilot study was conducted on ten healthy North American individuals who supplemented their diet with Immulina (R) (400 mg/day) for seven days. We observed a 40% average increase in the killing of K562 tumor cells by NK cells (p < 0.01) after Immulina (R) supplementation. In a separate placebo-controlled, crossover study involving 11 healthy Danish subjects, we observed increased mRNA expression of the NK cell marker NKG2D by 37% (p = 0.02) and by 55% (p = 0.0003) after administration of Immulina (R) (200 mg and 400 mg per day, respectively) for seven days. The mRNA expression of the NK- and T-cell marker perforin increased by 75% (p = 0.008) after administration of 400 mg Immulina (R) per day. Both markers displayed significant dose-dependent effects (p = 0.0003 and p = 0.02, respectively). The ratio between CD56(bright) and CD56(dim) NK cells was not affected by Immulina (R) administration. In summary, two independent studies showed enhancement of NK cell activity following administration of Immulina (R) for seven days.