T cell receptor-mediated negative selection of autoreactive T lymphocyte precursors occurs after commitment to the CD4 or CD8 lineages
T cell receptor-mediated negative selection of autoreactive T lymphocyte precursors occurs after commitment to the CD4 or CD8 lineages
复制标题
T 细胞受体介导的自身反应性 T 淋巴细胞前体的阴性选择发生在定型为 CD4 或 CD8 谱系之后
DOI：
--
复制
发表时间：
1990
影响因子：
15.3
通讯作者：
I L Weissman
中科院分区：
文献类型：
--
作者：
C J Guidos;J. S. Danska;C. G. Fathman;I L Weissman
为了鉴定T细胞受体（TCR）介导的阳性和阴性选择发生的成熟阶段，我们跟踪了在不同的阳性和阴性选择（主要组织相容性复合体或Mls）元件存在下来自TCR 10 CD 4 +8+胸腺母细胞的CD 4 +8-和CD 4 -8+ T细胞的发育。我们描述了新的谱系定向的过渡中间体，其是TCRmed CD 4 +8lo或TCRmed CD 4lo 8+，并且显示出已被积极选择的证据。此外，直到细胞已经获得TCR介导的过渡表型之一之后，负选择才是明显的。因此，我们提出，正常小鼠中的阴性选择仅在TCR 10 CD 4 +8+前体被阳性选择进入CD 4或CD 8谱系后发生。
To identify the maturational stage(s) during which T cell receptor (TCR)-mediated positive and negative selection occurs, we followed the development of CD4+8- and CD4-8+ T cells from TCRlo CD4+8+ thymic blasts in the presence of different positive and negative selecting (major histocompatibility complex or Mls) elements. We describe novel lineage-committed transitional intermediates that are TCRmed CD4+8lo or TCRmed CD4lo8+, and that show evidence of having been positively selected. Furthermore, negative selection is not evident until after cells have attained one of the TCRmed transitional phenotypes. Accordingly, we propose that negative selection in normal mice occurs only after TCRlo CD4+8+ precursors have been positively selected into either the CD4 or CD8 lineage.