Abundance and diversity of mucosa-associated hydrogenotrophic microbes in the healthy human colon
Abundance and diversity of mucosa-associated hydrogenotrophic microbes in the healthy human colon
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DOI：
10.1038/ismej.2011.90
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发表时间：
2012-01-01
期刊：
影响因子：
11
通讯作者：
Gaskins, H. Rex
中科院分区：
文献类型：
--
作者：
Nava, Gerardo M.;Carbonero, Franck;Gaskins, H. Rex
氢营养微生物群对结肠健康有显著影响;然而，对其多样性和原位生态知之甚少。在这里，基于分子的方法和多变量分析被用来检查的丰度和多样性的粘膜相关的氢营养微生物在90活检收集从右结肠，左结肠和直肠的25名健康受试者。在所有受试者的至少一个结肠区域中检测到所有三个氢营养组的功能基因。产甲烷古菌（MA）构成每个结肠区域中的氢营养微生物群的大约一半。在右半结肠中硫酸盐还原菌（SRB）比产乙酸菌丰富，而在左结肠和直肠中产乙酸菌比SRB丰富。MA基因型表现出较低的多样性，而SRB基因型是多样的，一般相似的三个地区内的主题，但显着变量之间的主题。多变量聚类分析定义的SRB基因型的多样性的主题特定的模式，但是，无论是主题或区域特定的集群，观察氢营养功能基因的丰度。功能基因克隆的序列分析显示，粘膜相关SRB与Desulfovibrio piger、Desulfovibrio sulfosecans和Bilophila wadsobella具有遗传学相关性;而MA与甲烷短杆菌属有关，MB. smithii和甲烷微生物目。总之，这些数据首次证明，人类结肠粘膜是持久的所有三组的氢营养微生物，表现出节段性和个体间的丰度和多样性的变化。The ISME Journal（2012）6，57-70; doi：10.1038/ismej.2011.90; 2011年7月14日在线发表
Hydrogenotrophic microbiota have a significant impact on colonic health; however, little is known about their diversity and ecology in situ. Here, molecular-based methods and multivariate analyses were used to examine the abundance and diversity of mucosa-associated hydrogenotrophic microbes in 90 biopsies collected from right colon, left colon and rectum of 25 healthy subjects. Functional genes of all three hydrogenotrophic groups were detected in at least one colonic region of all subjects. Methanogenic archaea (MA) constituted approximately one half of the hydrogenotrophic microbiota in each colonic region. Sulfate-reducing bacteria (SRB) were more abundant than acetogens in right colon, while acetogens were more abundant than SRB in left colon and rectum. MA genotypes exhibited low diversity, whereas SRB genotypes were diverse and generally similar across the three regions within subject but significantly variable among subjects. Multivariate cluster analysis defined subject-specific patterns for the diversity of SRB genotypes; however, neither subject-nor region-specific clusters were observed for the abundance of hydrogenotrophic functional genes. Sequence analyses of functional gene clones revealed that mucosa-associated SRB were phylogenetically related to Desulfovibrio piger, Desulfovibrio desulfuricans and Bilophila wadsworthia; whereas MA were related to Methanobrevibacter spp., Mb. smithii and the order Methanomicrobiales. Together these data demonstrate for the first time that the human colonic mucosa is persistently colonized by all three groups of hydrogenotrophic microbes, which exhibit segmental and interindividual variation in abundance and diversity. The ISME Journal (2012) 6, 57-70; doi:10.1038/ismej.2011.90; published online 14 July 2011