Fiber-optic biochemical gas sensor (bio-sniffer) for sub-ppb monitoring of formaldehyde vapor
Fiber-optic biochemical gas sensor (bio-sniffer) for sub-ppb monitoring of formaldehyde vapor
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DOI：
10.1016/j.snb.2011.10.065
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发表时间：
2012-01-03
影响因子：
8.4
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
--
作者：
Kudo, Hiroyuki;Wang, Xin;Mitsubayashi, Kohji
研制了一种高灵敏度的光纤生化气体传感器（生物嗅探器），用于甲醛（FA）蒸气的亚ppb级监测。生物嗅探器测量甲醛蒸汽作为还原烟酰胺腺嘌呤二核苷酸（NADH）的荧光，其是甲醛脱氢酶（NADH）反应的产物。采用峰值发射为335 nm的紫外发光二极管（UV-LED）作为激发光源。通过光纤将UV-LED的发射引入光电管，并在光电管处同轴拾取NADH的荧光。为了达到亚ppb的灵敏度，两种类型的检测器（光谱仪和光电倍增管（PMT））进行了测试。对反应体系进行了改进。首先测试了对甲醛更有活性的醛脱氢酶（ALDH），而不是ALDH。ALDH反应的产物再次被甲酸脱氢酶（FDH）氧化，以提高NADH的产量。作为这些改进的结果，实时监测FA进行了0.75 ppb的检测限，这是比较色谱方法。这种高灵敏度的监测方法有望提高室内空气管理的质量。(C)2011 Elsevier B. V.保留所有权利。
A high sensitive fiber-optic biochemical gas sensor (bio-sniffer) for sub-parts-per-billion (sub-ppb) monitoring of formaldehyde (FA) vapor was constructed and tested. The bio-sniffer measures formaldehyde vapor as fluorescence of reduced nicotinamide adenine dinucleotide (NADH), which is the product of formaldehyde dehydrogenase (FALDH) reaction. An ultraviolet light emitting diode (UV-LED) with peak emission of 335 nm was employed as an excitation light source. Emission of the UV-LED was introduced to the optode through an optical fiber and fluorescence of NADH was picked up coaxially at the optode. In order to achieve sub-ppb sensitivity, two types of detectors (a spectrometer and a photomultiplier tube (PMT)) were tested. The reaction system was also improved. Aldehyde dehydrogenase (ALDH), which is more active with formaldehyde, was first tested instead of FALDH. The product of ALDH reaction was again oxidized by formate dehydrogenase (FDH) to enhance production of NADH. As a result of these improvements, real-time monitoring of FA was performed with a detection limit of 0.75 ppb, which is comparative to chromatographic methods. Such a high sensitive monitoring method is expected to improve the quality of indoor air management. (C) 2011 Elsevier B.V. All rights reserved.